Metaphase karyotypes and G-banding in sandflies of the Lutzomyia longipalpis complex

Huaizhi Yin, J. P. Mutebi, S. Marriott, Gregory C Lanzaro

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Mitotic metaphase chromosomes (2n = 8) from brain cells of fourth instar sandfly larvae of four geographical strains of the Lutzomyia longipaplis complex were-examined microscopically, with bright-field illumination, after staining by a new G-banding technique involving exposure of air-dried chromosome preparations to quinacrine and ultraviolet light. Differences of G-banding and/or position of the centromere on chromosome 4 (the smallest chromosome pair) distinguished four putative sibling species from Costa Rica, Colombia and Brazil (distinctive populations from Jacobina and Lapinha Caves). The karyotype of the population from Jacobina, Brazil, showed an apparently plesiomorphic pattern of G-banding. On the basis of their recognizably different mitotic karyotypes, cytogenetic identification of separate taxa in the L. longipalpis complex should be useful for specific female vector competence and ecology studies.

Original languageEnglish (US)
Pages (from-to)72-77
Number of pages6
JournalMedical and Veterinary Entomology
Volume13
Issue number1
DOIs
StatePublished - Feb 1999
Externally publishedYes

Keywords

  • Brazil
  • Colombia
  • Costa Rica
  • Cytogenetics
  • G-banding
  • Genetics
  • Leishmaniasis vectors
  • Lutzomyia longipalpis
  • Mitotic metaphase karyotype
  • Sibling species

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

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