Metal ion inhibition of nonenzymatic pyridoxal phosphate catalyzed decarboxylation and transamination

R. F. Zabinski, M. D. Toney

Research output: Contribution to journalArticle

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Abstract

Nonenzymatic pyridoxal phosphate (PLP) catalyzed decarboxylations and transaminations have been revisited experimentally. Metal ions are known to catalyze a variety of PLP-dependent reactions in solution, including transamination. It is demonstrated here that the rate accelerations previously observed are due solely to enhancement of Schiff base formation under subsaturating conditions. A variety of metal ions were tested for their effects on the reactivity of the 2-methyl-2-aminomalonate Schiff bases. All were found to have either no effect or a small inhibitory one. The effects of Al3+ were studied in detail with the Schiff bases of 2-methyl-2-aminomalonate, 2-aminoisobutyrate, alanine, and ethylamine. The decarboxylation of 2-methyl-2-aminomalonate is unaffected by metalation with Al3+, while the decarboxylation of 2-aminoisobutyrate is inhibited 125-fold. The transamination reaction of ethylamine is 75-fold slower than that of alanine. Ethylamine transamination is inhibited 4-fold by Al3+ metalation, while alanine transamination is inhibited only 1.3-fold. Metal ion inhibition of Schiff base reactivity suggests a simple explanation for the lack of known PLP dependent enzymes that make direct mechanistic use of metal ions. A comparison of enzyme catalyzed, PLP catalyzed, and uncatalyzed reactions shows that PLP dependent decarboxylases are among the best known biological rate enhancers: decarboxylation occurs 1018-fold faster on the enzyme surface than it does free in solution. PLP itself provides the lion's share of the catalytic efficiency of the holoenzyme: at pH 8, free PLP catalyzes 2-aminoisobutyrate decarboxylation by ∼1010-fold, with the enzyme contributing an additional ∼108-fold.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalJournal of the American Chemical Society
Volume123
Issue number2
DOIs
StatePublished - Jan 17 2001

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Decarboxylation
Pyridoxal Phosphate
Metal ions
Phosphates
Metals
Ions
Schiff Bases
Enzymes
Alanine
Holoenzymes
Carboxy-Lyases
ethylamine

ASJC Scopus subject areas

  • Chemistry(all)

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Metal ion inhibition of nonenzymatic pyridoxal phosphate catalyzed decarboxylation and transamination. / Zabinski, R. F.; Toney, M. D.

In: Journal of the American Chemical Society, Vol. 123, No. 2, 17.01.2001, p. 193-198.

Research output: Contribution to journalArticle

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