Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells

Tao Yu, Yongtao Wang, Huizhen Zhang, Caroline H. Johnson, Yiming Jiang, Xiangjun Li, Zeming Wu, Tian Liu, Kristopher W. Krausz, Aiming Yu, Frank J. Gonzalez, Min Huang, Huichang Bi

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Mycoplasma contamination is a common problem in cell culture and can alter cellular functions. Since cell metabolism is either directly or indirectly involved in every aspect of cell function, it is important to detect changes to the cellular metabolome after mycoplasma infection. In this study, liquid chromatography mass spectrometry (LC/MS)-based metabolomics was used to investigate the effect of mycoplasma contamination on the cellular metabolism of human pancreatic carcinoma cells (PANC-1). Multivariate analysis demonstrated that mycoplasma contamination induced significant metabolic changes in PANC-1 cells. Twenty-three metabolites were identified and found to be involved in arginine and purine metabolism and energy supply. This study demonstrates that mycoplasma contamination significantly alters cellular metabolite levels, confirming the compelling need for routine checking of cell cultures for mycoplasma contamination, particularly when used for metabolomics studies. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)4267-4273
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume408
Issue number16
DOIs
StatePublished - Jun 1 2016

Keywords

  • Cell culture
  • Cellular metabolism
  • Metabolomics
  • Mycoplasma contamination
  • PANC-1 cells

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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    Yu, T., Wang, Y., Zhang, H., Johnson, C. H., Jiang, Y., Li, X., Wu, Z., Liu, T., Krausz, K. W., Yu, A., Gonzalez, F. J., Huang, M., & Bi, H. (2016). Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells. Analytical and Bioanalytical Chemistry, 408(16), 4267-4273. https://doi.org/10.1007/s00216-016-9525-9