Abstract
Rapamycin, known as an inhibitor of Target of Rapamycin (TOR), is an immunosuppressant drug used to prevent rejection in organ transplantation. Despite the close association of the TOR signaling cascade with various scopes of metabolism, it has not yet been thoroughly investigated at the metabolome level. In our current study, we applied mass spectrometric analysis for profiling primary metabolism in order to capture the responsive dynamics of the Chlamydomonas metabolome to the inhibition of TOR by rapamycin. Accordingly, we identified the impact of the rapamycin treatment at the level of metabolomic phenotypes that were clearly distinguished by multivariate statistical analysis. Pathway analysis pinpointed that inactivation of the TCA cycle was accompanied by the inhibition of cellular growth. Relative to the constant suppression of the TCA cycle, most amino acids were significantly increased in a time-dependent manner by longer exposure to rapamycin treatment, after an initial down-regulation at the early stage of exposure. Finally, we explored the isolation of the responsive metabolic factors into the rapamycin treatment and the culture duration, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 923-931 |
| Number of pages | 9 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 23 |
| Issue number | 7 |
| DOIs | |
| State | Published - 2013 |
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Keywords
- Chlamydomonas reinhardtii
- Mass spectrometry
- Metabolomics
- Target of rapamycin
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Metabolomic response of chlamydomonas reinhardtii to the s inhibition of target of rapamycin (tor) by rapamycin. / Lee, Do Yup; Fiehn, Oliver.
In: Journal of Microbiology and Biotechnology, Vol. 23, No. 7, 2013, p. 923-931.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolomic response of chlamydomonas reinhardtii to the s inhibition of target of rapamycin (tor) by rapamycin
AU - Lee, Do Yup
AU - Fiehn, Oliver
PY - 2013
Y1 - 2013
N2 - Rapamycin, known as an inhibitor of Target of Rapamycin (TOR), is an immunosuppressant drug used to prevent rejection in organ transplantation. Despite the close association of the TOR signaling cascade with various scopes of metabolism, it has not yet been thoroughly investigated at the metabolome level. In our current study, we applied mass spectrometric analysis for profiling primary metabolism in order to capture the responsive dynamics of the Chlamydomonas metabolome to the inhibition of TOR by rapamycin. Accordingly, we identified the impact of the rapamycin treatment at the level of metabolomic phenotypes that were clearly distinguished by multivariate statistical analysis. Pathway analysis pinpointed that inactivation of the TCA cycle was accompanied by the inhibition of cellular growth. Relative to the constant suppression of the TCA cycle, most amino acids were significantly increased in a time-dependent manner by longer exposure to rapamycin treatment, after an initial down-regulation at the early stage of exposure. Finally, we explored the isolation of the responsive metabolic factors into the rapamycin treatment and the culture duration, respectively.
AB - Rapamycin, known as an inhibitor of Target of Rapamycin (TOR), is an immunosuppressant drug used to prevent rejection in organ transplantation. Despite the close association of the TOR signaling cascade with various scopes of metabolism, it has not yet been thoroughly investigated at the metabolome level. In our current study, we applied mass spectrometric analysis for profiling primary metabolism in order to capture the responsive dynamics of the Chlamydomonas metabolome to the inhibition of TOR by rapamycin. Accordingly, we identified the impact of the rapamycin treatment at the level of metabolomic phenotypes that were clearly distinguished by multivariate statistical analysis. Pathway analysis pinpointed that inactivation of the TCA cycle was accompanied by the inhibition of cellular growth. Relative to the constant suppression of the TCA cycle, most amino acids were significantly increased in a time-dependent manner by longer exposure to rapamycin treatment, after an initial down-regulation at the early stage of exposure. Finally, we explored the isolation of the responsive metabolic factors into the rapamycin treatment and the culture duration, respectively.
KW - Chlamydomonas reinhardtii
KW - Mass spectrometry
KW - Metabolomics
KW - Target of rapamycin
UR - http://www.scopus.com/inward/record.url?scp=84880677727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880677727&partnerID=8YFLogxK
U2 - /10.4014/jmb.1304.04057
DO - /10.4014/jmb.1304.04057
M3 - Article
C2 - 23727803
AN - SCOPUS:84880677727
VL - 23
SP - 923
EP - 931
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 7
ER -