Metabolomic response of chlamydomonas reinhardtii to the s inhibition of target of rapamycin (tor) by rapamycin

Do Yup Lee, Oliver Fiehn

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Rapamycin, known as an inhibitor of Target of Rapamycin (TOR), is an immunosuppressant drug used to prevent rejection in organ transplantation. Despite the close association of the TOR signaling cascade with various scopes of metabolism, it has not yet been thoroughly investigated at the metabolome level. In our current study, we applied mass spectrometric analysis for profiling primary metabolism in order to capture the responsive dynamics of the Chlamydomonas metabolome to the inhibition of TOR by rapamycin. Accordingly, we identified the impact of the rapamycin treatment at the level of metabolomic phenotypes that were clearly distinguished by multivariate statistical analysis. Pathway analysis pinpointed that inactivation of the TCA cycle was accompanied by the inhibition of cellular growth. Relative to the constant suppression of the TCA cycle, most amino acids were significantly increased in a time-dependent manner by longer exposure to rapamycin treatment, after an initial down-regulation at the early stage of exposure. Finally, we explored the isolation of the responsive metabolic factors into the rapamycin treatment and the culture duration, respectively.

Original languageEnglish (US)
Pages (from-to)923-931
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume23
Issue number7
DOIs
StatePublished - 2013

Keywords

  • Chlamydomonas reinhardtii
  • Mass spectrometry
  • Metabolomics
  • Target of rapamycin

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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