Abstract
Metabolomics is one of the newcomers among the "omics" techniques, perhaps also constituting the most relevant for the study of pathophysiological conditions. Metabolomics may indeed yield not only disease-specific biomarkers but also profound insights into the etiology and progression of a variety of human disorders. Various metabolomic approaches are currently available to study oncogenesis and tumor progression in vivo, in murine tumor models. Many of these models rely on the xenograft of human cancer cells into immunocompromised mice. Understanding how the metabolism of these cells evolves in vivo is critical to evaluate the actual pertinence of xenograft models to human pathology. Here, we discuss various tumor xenograft models and methods for their metabolomic profiling to provide a short guide to investigators interested in this field of research.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Enzymology |
| Publisher | Academic Press Inc. |
| Pages | 275-296 |
| Number of pages | 22 |
| Volume | 543 |
| ISBN (Print) | 9780128013298 |
| DOIs | |
| State | Published - 2014 |
Publication series
| Name | Methods in Enzymology |
|---|---|
| Volume | 543 |
| ISSN (Print) | 00766879 |
| ISSN (Electronic) | 15577988 |
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Keywords
- Biomarkers
- Mass spectrometry
- Metabolomics
- Mouse models
- Oncology
- Xenograft
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Metabolomic profiling of tumor-bearing mice. / Wettersten, Hiromi I.; Ganti, Sheila; Weiss, Robert H.
Methods in Enzymology. Vol. 543 Academic Press Inc., 2014. p. 275-296 (Methods in Enzymology; Vol. 543).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Metabolomic profiling of tumor-bearing mice
AU - Wettersten, Hiromi I.
AU - Ganti, Sheila
AU - Weiss, Robert H
PY - 2014
Y1 - 2014
N2 - Metabolomics is one of the newcomers among the "omics" techniques, perhaps also constituting the most relevant for the study of pathophysiological conditions. Metabolomics may indeed yield not only disease-specific biomarkers but also profound insights into the etiology and progression of a variety of human disorders. Various metabolomic approaches are currently available to study oncogenesis and tumor progression in vivo, in murine tumor models. Many of these models rely on the xenograft of human cancer cells into immunocompromised mice. Understanding how the metabolism of these cells evolves in vivo is critical to evaluate the actual pertinence of xenograft models to human pathology. Here, we discuss various tumor xenograft models and methods for their metabolomic profiling to provide a short guide to investigators interested in this field of research.
AB - Metabolomics is one of the newcomers among the "omics" techniques, perhaps also constituting the most relevant for the study of pathophysiological conditions. Metabolomics may indeed yield not only disease-specific biomarkers but also profound insights into the etiology and progression of a variety of human disorders. Various metabolomic approaches are currently available to study oncogenesis and tumor progression in vivo, in murine tumor models. Many of these models rely on the xenograft of human cancer cells into immunocompromised mice. Understanding how the metabolism of these cells evolves in vivo is critical to evaluate the actual pertinence of xenograft models to human pathology. Here, we discuss various tumor xenograft models and methods for their metabolomic profiling to provide a short guide to investigators interested in this field of research.
KW - Biomarkers
KW - Mass spectrometry
KW - Metabolomics
KW - Mouse models
KW - Oncology
KW - Xenograft
UR - http://www.scopus.com/inward/record.url?scp=84902206267&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902206267&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-801329-8.00014-3
DO - 10.1016/B978-0-12-801329-8.00014-3
M3 - Chapter
C2 - 24924138
AN - SCOPUS:84902206267
SN - 9780128013298
VL - 543
T3 - Methods in Enzymology
SP - 275
EP - 296
BT - Methods in Enzymology
PB - Academic Press Inc.
ER -