Metabolomic phenotyping validates the infant rhesus monkey as a model of human infant metabolism

Aifric O'Sullivan, Xuan He, Elizabeth M S McNiven, Katie Hinde, Neill W. Haggarty, Bo Lönnerdal, Carolyn M. Slupsky

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

OBJECTIVES: Rhesus macaque monkeys are widely used as models for human physiology and behavior. They are particularly suited for studies on infant nutrition and metabolism; however, few studies have directly compared their metabolic or microbiological phenotypes. The aim of the present study was to compare the metabolomic profiles and microbiome of milk from human and rhesus mothers, and the metabolomic profiles of urine and serum from human and rhesus infants to establish the value of this model for human nutrition research. METHODS: Milk samples were collected from rhesus and human mothers at similar stages of lactation. Urine and serum samples were collected from breast-fed rhesus and human infants. 1H nuclear magnetic resonance spectra were acquired for all samples and metabolites were identified and quantified using targeted profiling techniques. The microbial community structure of milk was examined using 16S rRNA gene sequencing. RESULTS: An identical set of metabolites was identified in the urine and serum profiles from human and rhesus infants. In urine, 65% of the metabolites were present at similar concentrations, whereas ∼40% were similar in serum. The gross composition of human and rhesus milk was comparable, including the overall microbial community at both the phylum and order level; however, some oligosaccharides found in human milk were not present in monkey milk. CONCLUSIONS: Comparison of the milk microbiome and urine, serum, and milk metabolome of rhesus macaques and humans has revealed substantial similarities that provide unique biological information highlighting the significance of rhesus macaques as a model for infant nutrition and developmental research.

Original languageEnglish (US)
Pages (from-to)355-363
Number of pages9
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume56
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

Metabolomics
Macaca mulatta
Milk
Urine
Human Milk
Serum
Microbiota
Mothers
Metabolome
Oligosaccharides
rRNA Genes
Lactation
Research
Haplorhini
Breast
Magnetic Resonance Spectroscopy
Phenotype

Keywords

  • H NMR spectroscopy
  • infant nutrition
  • metabolomics
  • milk microbiome
  • rhesus macaque

ASJC Scopus subject areas

  • Gastroenterology
  • Pediatrics, Perinatology, and Child Health

Cite this

O'Sullivan, A., He, X., McNiven, E. M. S., Hinde, K., Haggarty, N. W., Lönnerdal, B., & Slupsky, C. M. (2013). Metabolomic phenotyping validates the infant rhesus monkey as a model of human infant metabolism. Journal of Pediatric Gastroenterology and Nutrition, 56(4), 355-363. https://doi.org/10.1097/MPG.0b013e31827e1f07

Metabolomic phenotyping validates the infant rhesus monkey as a model of human infant metabolism. / O'Sullivan, Aifric; He, Xuan; McNiven, Elizabeth M S; Hinde, Katie; Haggarty, Neill W.; Lönnerdal, Bo; Slupsky, Carolyn M.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 56, No. 4, 04.2013, p. 355-363.

Research output: Contribution to journalArticle

O'Sullivan, A, He, X, McNiven, EMS, Hinde, K, Haggarty, NW, Lönnerdal, B & Slupsky, CM 2013, 'Metabolomic phenotyping validates the infant rhesus monkey as a model of human infant metabolism', Journal of Pediatric Gastroenterology and Nutrition, vol. 56, no. 4, pp. 355-363. https://doi.org/10.1097/MPG.0b013e31827e1f07
O'Sullivan, Aifric ; He, Xuan ; McNiven, Elizabeth M S ; Hinde, Katie ; Haggarty, Neill W. ; Lönnerdal, Bo ; Slupsky, Carolyn M. / Metabolomic phenotyping validates the infant rhesus monkey as a model of human infant metabolism. In: Journal of Pediatric Gastroenterology and Nutrition. 2013 ; Vol. 56, No. 4. pp. 355-363.
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