Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae

Theodore R. Sana, Steve Fischer, Gert Wohlgemuth, Anjali Katrekar, Ki hong Jung, Pam C. Ronald, Oliver Fiehn

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo), gives rise to devastating crop losses in rice. Disease resistant rice cultivars are the most economical way to combat the disease. The TP309 cultivar is susceptible to infection by Xoo strain PXO99. A transgenic variety, TP309_Xa21, expresses the pattern recognition receptor Xa21, and is resistant. PXO99△raxST, a strain lacking the raxST gene, is able to overcome Xa21-mediated immunity. We used a single extraction solvent to demonstrate comprehensive metabolomics and transcriptomics profiling under sample limited conditions, and analyze the molecular responses of two rice lines challenged with either PXO99 or PXO99△raxST. LC-TOF raw data file filtering resulted in better within group reproducibility of replicate samples for statistical analyses. Accurate mass match compound identification with molecular formula generation (MFG) ranking of 355 masses was achieved with the METLIN database. GC-TOF analysis yielded an additional 441 compounds after BinBase database processing, of which 154 were structurally identified by retention index/MS library matching. Multivariate statistics revealed that the susceptible and resistant genotypes possess distinct profiles. Although few mRNA and metabolite differences were detected in PXO99 challenged TP309 compared to mock, many differential changes occurred in the Xa21-mediated response to PXO99 and PXO99△raxST. Acetophenone, xanthophylls, fatty acids, alkaloids, glutathione, carbohydrate and lipid biosynthetic pathways were affected. Significant transcriptional induction of several pathogenesis related genes in Xa21 challenged strains, as well as differential changes to GAD, PAL, ICL1 and Glutathione-S-transferase transcripts indicated limited correlation with metabolite changes under single time point global profiling conditions.

Original languageEnglish (US)
Pages (from-to)451-465
Number of pages15
JournalMetabolomics
Volume6
Issue number3
DOIs
StatePublished - 2010

Fingerprint

Xanthomonas
Metabolomics
Pathogens
Metabolites
Genes
Xanthophylls
Pattern Recognition Receptors
Solvent extraction
Glutathione Transferase
Alkaloids
Crops
Glutathione
Databases
Fatty Acids
Carbohydrates
Statistics
Information Storage and Retrieval
Biosynthetic Pathways
Lipids
Messenger RNA

Keywords

  • Bacterial leaf blight
  • GC/MS
  • LC/MS
  • Metabolomics
  • Microarray
  • Pathogen
  • Pathogen associated molecular pattern
  • Pattern recognition receptor
  • Rice

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Sana, T. R., Fischer, S., Wohlgemuth, G., Katrekar, A., Jung, K. H., Ronald, P. C., & Fiehn, O. (2010). Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. Metabolomics, 6(3), 451-465. https://doi.org/10.1007/s11306-010-0218-7

Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. / Sana, Theodore R.; Fischer, Steve; Wohlgemuth, Gert; Katrekar, Anjali; Jung, Ki hong; Ronald, Pam C.; Fiehn, Oliver.

In: Metabolomics, Vol. 6, No. 3, 2010, p. 451-465.

Research output: Contribution to journalArticle

Sana, TR, Fischer, S, Wohlgemuth, G, Katrekar, A, Jung, KH, Ronald, PC & Fiehn, O 2010, 'Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae', Metabolomics, vol. 6, no. 3, pp. 451-465. https://doi.org/10.1007/s11306-010-0218-7
Sana, Theodore R. ; Fischer, Steve ; Wohlgemuth, Gert ; Katrekar, Anjali ; Jung, Ki hong ; Ronald, Pam C. ; Fiehn, Oliver. / Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. In: Metabolomics. 2010 ; Vol. 6, No. 3. pp. 451-465.
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