Metabolizable 111in chelate conjugated anti-idiotype monoclonal antibody for radioimmunodetection of lymphoma in mice

Michael K. Haseman, David A. Goodwin, Claude F. Meares, Mark S. Kaminski, Theodore G. Wensel, Michael J. McCall, Ronald Levy

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

The relative biological properties of 111In-labeled monoclonal antibodies (MoAb) coupled with a conventional bifunctional chelate (BC) and a new, enzyme metabolizable, bifunctional chelate (BCM) were investigated. A rat IgG2a MoAb against idiotype from a mouse B-cell lymphoma was utilized. Mice bearing B-cell lymphomas in the subcutaneous tissues of the flank were given IV-injections of labeled MoAb and imaged or killed for organ counting at 24 h or 48 h. Rat anti-dinitrophenyl IgG2a MoAb and non-specific polyclonal mouse IgG were used as controls. Compared to BC, the use of BCM resulted in a substantial decrease in blood background activity, a shorter biological half-life and an increase in tumor to blood ratio at the expense of a moderate decrease in absolute tumor uptake. The versatile chemistry of these C-1 substituted bifunctional chelates provides a variety of possible enzyme cleavable moieties for further investigation.

Original languageEnglish (US)
Pages (from-to)455-460
Number of pages6
JournalEuropean Journal Of Nuclear Medicine
Volume12
Issue number9
DOIs
StatePublished - Dec 1986

Keywords

  • B-cell lymphoma
  • Monoclonal antibody
  • Radionuclide imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Haseman, M. K., Goodwin, D. A., Meares, C. F., Kaminski, M. S., Wensel, T. G., McCall, M. J., & Levy, R. (1986). Metabolizable 111in chelate conjugated anti-idiotype monoclonal antibody for radioimmunodetection of lymphoma in mice. European Journal Of Nuclear Medicine, 12(9), 455-460. https://doi.org/10.1007/BF00254750