Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity

Christopher J. Botanga, Gerit Bethke, Zhong Chen, Daniel R. Gallie, Oliver Fiehn, Jane Glazebrook

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The interaction between the pathogenic ascomycete Alternaria brassicicola and Arabidopsis was investigated by metabolite profiling. The effect of A. brassicicola challenge on metabolite levels was substantial, with nearly 50% of detected compounds undergoing significant changes. Mutations blocking ethylene, jasmonic acid, or ethylene signaling had little effect on metabolite levels. The effects of altering levels of some metabolites were tested by exogenous application during A. brassicicola inoculation. Gamma amino-butyric acid (GABA) or xylitol promoted, while trehalose and ascorbate inhibited, disease severity. GABA promoted, and ascorbate strongly inhibited, fungal growth in culture. Arabidopsis vtc1 and vtc2 mutants, that have low levels of ascorbate, were more susceptible to A. brassicicola. Ascorbate levels declined following A. brassicicola inoculation while levels of dehydroascorbate increased, resulting in a shift of the redox balance between these compounds in the direction of oxidation. These results demonstrate that ascorbate is an important component of resistance to this pathogen.

Original languageEnglish (US)
Pages (from-to)1628-1638
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume25
Issue number12
DOIs
StatePublished - Dec 2012

Fingerprint

Alternaria brassicicola
Alternaria
Butyric Acid
Arabidopsis
disease severity
metabolites
Xylitol
Ascomycota
Trehalose
Oxidation-Reduction
gamma-aminobutyric acid
ethylene
Mutation
Growth
xylitol
trehalose
jasmonic acid
microbial growth
oxidation
mutation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity. / Botanga, Christopher J.; Bethke, Gerit; Chen, Zhong; Gallie, Daniel R.; Fiehn, Oliver; Glazebrook, Jane.

In: Molecular Plant-Microbe Interactions, Vol. 25, No. 12, 12.2012, p. 1628-1638.

Research output: Contribution to journalArticle

Botanga, Christopher J. ; Bethke, Gerit ; Chen, Zhong ; Gallie, Daniel R. ; Fiehn, Oliver ; Glazebrook, Jane. / Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity. In: Molecular Plant-Microbe Interactions. 2012 ; Vol. 25, No. 12. pp. 1628-1638.
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