Metabolite profiling in Arabidopsis.

Oliver Fiehn

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Metabolite profiling is the multiparallel relative quantification of a mixture of compounds or compound classes using chromatography and universal detection technologies (GC-MS, LC-MS). In this respect it is an extension of classical single-target methods from which it can be distinguished by its broader view on profiling major biochemical events. This broader scope of analysis outweighs the disadvantages by making compromises in method development and the reduced accuracy for specific metabolites. This chapter exemplifies the strategies in metabolite profiling of polar compounds by gas chromatography-mass spectrometry (GC-MS). It gives experimental details on the basic steps: harvest, homogenization, extraction, fractionation, concentration, derivatization, data acquisition, raw data processing and result data tranformation.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume323
StatePublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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