Metabolite content profiling of bottlenose dolphin exhaled breath

Alexander A. Aksenov, Laura Yeates, Alberto Pasamontes, Craig Siebe, Yuriy Zrodnikov, Jason Simmons, Mitchell M. McCartney, Jean Pierre Deplanque, Randall S. Wells, Cristina E Davis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Changing ocean health and the potential impact on marine mammal health are gaining global attention. Direct health assessments of wild marine mammals, however, is inherently difficult. Breath analysis metabolomics is a very attractive assessment tool due to its noninvasive nature, but it is analytically challenging. It has never been attempted in cetaceans for comprehensive metabolite profiling. We have developed a method to reproducibly sample breath from small cetaceans, specifically Atlantic bottlenose dolphins (Tursiops truncatus). We describe the analysis workflow to profile exhaled breath metabolites and provide here a first library of volatile and nonvolatile compounds in cetacean exhaled breath. The described analytical methodology enabled us to document baseline compounds in exhaled breath of healthy animals and to study changes in metabolic content of dolphin breath with regard to a variety of factors. The method of breath analysis may provide a very valuable tool in future wildlife conservation efforts as well as deepen our understanding of marine mammals biology and physiology.

Original languageEnglish (US)
Pages (from-to)10616-10624
Number of pages9
JournalAnalytical Chemistry
Volume86
Issue number21
DOIs
StatePublished - Sep 25 2014

Fingerprint

Mammals
Metabolites
Health
Physiology
Conservation
Animals

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Aksenov, A. A., Yeates, L., Pasamontes, A., Siebe, C., Zrodnikov, Y., Simmons, J., ... Davis, C. E. (2014). Metabolite content profiling of bottlenose dolphin exhaled breath. Analytical Chemistry, 86(21), 10616-10624. https://doi.org/10.1021/ac5024217

Metabolite content profiling of bottlenose dolphin exhaled breath. / Aksenov, Alexander A.; Yeates, Laura; Pasamontes, Alberto; Siebe, Craig; Zrodnikov, Yuriy; Simmons, Jason; McCartney, Mitchell M.; Deplanque, Jean Pierre; Wells, Randall S.; Davis, Cristina E.

In: Analytical Chemistry, Vol. 86, No. 21, 25.09.2014, p. 10616-10624.

Research output: Contribution to journalArticle

Aksenov, AA, Yeates, L, Pasamontes, A, Siebe, C, Zrodnikov, Y, Simmons, J, McCartney, MM, Deplanque, JP, Wells, RS & Davis, CE 2014, 'Metabolite content profiling of bottlenose dolphin exhaled breath', Analytical Chemistry, vol. 86, no. 21, pp. 10616-10624. https://doi.org/10.1021/ac5024217
Aksenov AA, Yeates L, Pasamontes A, Siebe C, Zrodnikov Y, Simmons J et al. Metabolite content profiling of bottlenose dolphin exhaled breath. Analytical Chemistry. 2014 Sep 25;86(21):10616-10624. https://doi.org/10.1021/ac5024217
Aksenov, Alexander A. ; Yeates, Laura ; Pasamontes, Alberto ; Siebe, Craig ; Zrodnikov, Yuriy ; Simmons, Jason ; McCartney, Mitchell M. ; Deplanque, Jean Pierre ; Wells, Randall S. ; Davis, Cristina E. / Metabolite content profiling of bottlenose dolphin exhaled breath. In: Analytical Chemistry. 2014 ; Vol. 86, No. 21. pp. 10616-10624.
@article{c03034f908f5419f9a2c7e6ae92ca966,
title = "Metabolite content profiling of bottlenose dolphin exhaled breath",
abstract = "Changing ocean health and the potential impact on marine mammal health are gaining global attention. Direct health assessments of wild marine mammals, however, is inherently difficult. Breath analysis metabolomics is a very attractive assessment tool due to its noninvasive nature, but it is analytically challenging. It has never been attempted in cetaceans for comprehensive metabolite profiling. We have developed a method to reproducibly sample breath from small cetaceans, specifically Atlantic bottlenose dolphins (Tursiops truncatus). We describe the analysis workflow to profile exhaled breath metabolites and provide here a first library of volatile and nonvolatile compounds in cetacean exhaled breath. The described analytical methodology enabled us to document baseline compounds in exhaled breath of healthy animals and to study changes in metabolic content of dolphin breath with regard to a variety of factors. The method of breath analysis may provide a very valuable tool in future wildlife conservation efforts as well as deepen our understanding of marine mammals biology and physiology.",
author = "Aksenov, {Alexander A.} and Laura Yeates and Alberto Pasamontes and Craig Siebe and Yuriy Zrodnikov and Jason Simmons and McCartney, {Mitchell M.} and Deplanque, {Jean Pierre} and Wells, {Randall S.} and Davis, {Cristina E}",
year = "2014",
month = "9",
day = "25",
doi = "10.1021/ac5024217",
language = "English (US)",
volume = "86",
pages = "10616--10624",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "21",

}

TY - JOUR

T1 - Metabolite content profiling of bottlenose dolphin exhaled breath

AU - Aksenov, Alexander A.

AU - Yeates, Laura

AU - Pasamontes, Alberto

AU - Siebe, Craig

AU - Zrodnikov, Yuriy

AU - Simmons, Jason

AU - McCartney, Mitchell M.

AU - Deplanque, Jean Pierre

AU - Wells, Randall S.

AU - Davis, Cristina E

PY - 2014/9/25

Y1 - 2014/9/25

N2 - Changing ocean health and the potential impact on marine mammal health are gaining global attention. Direct health assessments of wild marine mammals, however, is inherently difficult. Breath analysis metabolomics is a very attractive assessment tool due to its noninvasive nature, but it is analytically challenging. It has never been attempted in cetaceans for comprehensive metabolite profiling. We have developed a method to reproducibly sample breath from small cetaceans, specifically Atlantic bottlenose dolphins (Tursiops truncatus). We describe the analysis workflow to profile exhaled breath metabolites and provide here a first library of volatile and nonvolatile compounds in cetacean exhaled breath. The described analytical methodology enabled us to document baseline compounds in exhaled breath of healthy animals and to study changes in metabolic content of dolphin breath with regard to a variety of factors. The method of breath analysis may provide a very valuable tool in future wildlife conservation efforts as well as deepen our understanding of marine mammals biology and physiology.

AB - Changing ocean health and the potential impact on marine mammal health are gaining global attention. Direct health assessments of wild marine mammals, however, is inherently difficult. Breath analysis metabolomics is a very attractive assessment tool due to its noninvasive nature, but it is analytically challenging. It has never been attempted in cetaceans for comprehensive metabolite profiling. We have developed a method to reproducibly sample breath from small cetaceans, specifically Atlantic bottlenose dolphins (Tursiops truncatus). We describe the analysis workflow to profile exhaled breath metabolites and provide here a first library of volatile and nonvolatile compounds in cetacean exhaled breath. The described analytical methodology enabled us to document baseline compounds in exhaled breath of healthy animals and to study changes in metabolic content of dolphin breath with regard to a variety of factors. The method of breath analysis may provide a very valuable tool in future wildlife conservation efforts as well as deepen our understanding of marine mammals biology and physiology.

UR - http://www.scopus.com/inward/record.url?scp=84908568718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908568718&partnerID=8YFLogxK

U2 - 10.1021/ac5024217

DO - 10.1021/ac5024217

M3 - Article

C2 - 25254551

AN - SCOPUS:84908568718

VL - 86

SP - 10616

EP - 10624

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 21

ER -