Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes: Studies with gas chromatography-mass spectrometry

M. Yudkoff, I. Nissim, S. U. Kim, David E Pleasure, Stanton Segal

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.

Original languageEnglish (US)
Pages (from-to)283-286
Number of pages4
JournalJournal of Neurochemistry
Volume42
Issue number1
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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