Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes: Studies with gas chromatography-mass spectrometry

M. Yudkoff, I. Nissim, S. U. Kim, David E Pleasure, Stanton Segal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.

Original languageEnglish (US)
Pages (from-to)283-286
Number of pages4
JournalJournal of Neurochemistry
Volume42
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

Metabolism
Astrocytes
Gas chromatography
Gas Chromatography-Mass Spectrometry
Labeling
Mass spectrometry
Glutamine
Aspartic Acid
Amides
Alanine
Glycine
Serine
Glutamic Acid
Monolayers

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes : Studies with gas chromatography-mass spectrometry. / Yudkoff, M.; Nissim, I.; Kim, S. U.; Pleasure, David E; Segal, Stanton.

In: Journal of Neurochemistry, Vol. 42, No. 1, 1984, p. 283-286.

Research output: Contribution to journalArticle

@article{49dfdfb4eda74eb9b1b716af6f3afe15,
title = "Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes: Studies with gas chromatography-mass spectrometry",
abstract = "Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.",
author = "M. Yudkoff and I. Nissim and Kim, {S. U.} and Pleasure, {David E} and Stanton Segal",
year = "1984",
language = "English (US)",
volume = "42",
pages = "283--286",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes

T2 - Studies with gas chromatography-mass spectrometry

AU - Yudkoff, M.

AU - Nissim, I.

AU - Kim, S. U.

AU - Pleasure, David E

AU - Segal, Stanton

PY - 1984

Y1 - 1984

N2 - Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.

AB - Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.

UR - http://www.scopus.com/inward/record.url?scp=0021288355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021288355&partnerID=8YFLogxK

M3 - Article

C2 - 6689695

AN - SCOPUS:0021288355

VL - 42

SP - 283

EP - 286

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 1

ER -