Metabolism of indium chelates attached to monoclonal antibody: Minimal transchelation of indium from benzyl-EDTA chelate in vivo

S. V. Deshpande, R. Subramanian, M. J. McCall, S. J. DeNardo, Gerald L Denardo, C. F. Meares

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

The metabolism of radiolabeled antibodies is important for radioimmunoimaging and therapy. The loss of indium-111 (111In) from the chelate can pose problems in imaging and increases the radiation dose to normal tissues. We have evaluated the loss in vivo of 111In from Lym-1-benzyl-EDTA-111In (an antibody conjugated with isothiocyanatobenzyl-EDTA) in normal mice. A monoclonal antibody (CHA 255) that binds to benzyl-EDTA-indium chelates, but not to other forms of indium, was used to measure the percent of 111In remaining in the chelate. Four days after injection, 97.4 ± 2.2% of the 111In in the liver was still in the benzyl-EDTA chelate, as was 99.4 ± 0.7% of the 111In in the urine, and 99.1 ± 0.7% of the 111In in the blood. Studies in vitro indicate that a benzyl-EDTA-111In-antibody-chelate conjugate is more stable in human serum than a benzyl-DTPA-111In conjugate, and that both benzyl-chelate conjugates are much more stable than an unsubstituted DTPA conjugate.

Original languageEnglish (US)
Pages (from-to)218-224
Number of pages7
JournalJournal of Nuclear Medicine
Volume31
Issue number2
StatePublished - 1990

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

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