Metabolic variations in different citrus rootstock cultivars associated with different responses to Huanglongbing

Ute Albrecht, Oliver Fiehn, Kim D. Bowman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Huanglongbing (HLB) is one of the most destructive bacterial diseases of citrus. No resistant cultivars have been identified, although tolerance has been observed in the genus Poncirus and some of its hybrids with Citrus that are commonly used as rootstocks. In this study we exploited this tolerance by comparing five different tolerant hybrids with a cultivar that shows pronounced HLB sensitivity to discern potential contributing metabolic factors. Whole leaves of infected and non-infected greenhouse-grown seedlings were extracted and subjected to untargeted GC-TOF MS based metabolomics. After BinBase data filtering, 342 (experiment 1) and 650 (experiment 2) unique metabolites were quantified, of which 122 and 195, respectively, were assigned by chemical structures. The number of metabolites found to be differently regulated in the infected state compared with the non-infected state varied between the cultivars and was largest (166) in the susceptible cultivar Cleopatra mandarin (Citrus reticulata) and lowest (3) in the tolerant cultivars US-897 (C. reticulata 'Cleopatra' × Poncirus trifoliata) and US-942 (C. reticulata 'Sunki' × P. trifoliata) from experiment 2. Tolerance to HLB did not appear to be associated with accumulation of higher amounts of protective metabolites in response to infection. Many metabolites were found in higher concentrations in the tolerant cultivars compared with susceptible Cleopatra mandarin and may play important roles in conferring tolerance to HLB. Lower availability of specific sugars necessary for survival of the pathogen may also be a contributing factor in the decreased disease severity observed for these cultivars.

Original languageEnglish (US)
Pages (from-to)33-44
Number of pages12
JournalPlant Physiology and Biochemistry
Volume107
DOIs
StatePublished - Oct 1 2016

Fingerprint

greening disease
Citrus
rootstocks
Poncirus
cultivars
Citrus reticulata
metabolites
Poncirus trifoliata
mandarins
Metabolomics
Seedlings
pathogen survival
metabolomics
chemical structure
disease severity
Infection
sugars
greenhouses
seedlings
infection

Keywords

  • Candidatus Liberibacter asiaticus
  • Citrus
  • Cultivars
  • Gas chromatography mass spectrometry
  • HLB
  • Metabolites
  • Rootstock

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Metabolic variations in different citrus rootstock cultivars associated with different responses to Huanglongbing. / Albrecht, Ute; Fiehn, Oliver; Bowman, Kim D.

In: Plant Physiology and Biochemistry, Vol. 107, 01.10.2016, p. 33-44.

Research output: Contribution to journalArticle

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