Metabolic regulation of α-amylase gene expression in transgenic cell cultures of rice (Oryza sativa L.)

Ning Huang, John Chandler, Bruce R. Thomas, Nozomu Koizumi, Raymond L. Rodriguez

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Expression of two genes in the α-amylase gene family is controlled by metabolic regulation in rice cultured cells. The levels of RAmy3D and RAmy3E mRNAs in rice cultured cells are inversely related to the concentration of sugar in the culture medium. Other genes in the rice α-amylase gene family have little or no expression in cultured cells; these expression levels are not controlled by metabolic regulation. A RAmy3D promoter/GUS gene fusion was metabolically regulated in the transgenic rice cell line 3DG, just as the endogenous RAmy3D gene is regulated. An assay of GUS enzyme activity in 3DG cells demonstrated that RAmy3D/GUS expression is repressed when sugar is present in the culture medium and induced when sugar is removed from the medium. The 942 bp fragment of the RAmy3D promoter that was linked to the coding region of the GUS reporter gene thus contains all of the regulatory sequences necessary for metabolic regulation of the gene.

Original languageEnglish (US)
Pages (from-to)737-747
Number of pages11
JournalPlant Molecular Biology
Volume23
Issue number4
DOIs
StatePublished - Nov 1993

Keywords

  • α-amylase
  • gene expression
  • metabolic regulation
  • Oryza sativa
  • transcription
  • transgenic cell culture

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

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