Metabolic fluctuation during a muscle contraction cycle

Youngran Chung; Robert Sharman; Richard Carlsen; Steven W. Unger; Douglas Larson; Thomas Jue

Metabolic fluctuation during a muscle contraction cycle

Youngran Chung, Robert Sharman, Richard Carlsen, Steven W. Unger, Douglas Larson, Thomas Jue

Biochemistry and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Gated ³¹P-nuclear magnetic resonance followed the metabolic fluctuation in rat gastrocnemius muscle during a contraction cycle. Within 16 ms after stimulation, the phosphocreatine (PCr) level drops 11.3% from its reference state. The PCr minimum corresponds closely to the time of maximum force contraction. P(i) increases stoichiometrically, while ATP remains constant. During a twitch, PCr hydrolysis produces 3.1 μmol ATP/g tissue, which is substantially higher than the reported 0.3 μmol ATP·twitch^-·g tissue^-1 derived from steady-state experiments. The results reveal that a substantial energy fluctuation accompanies a muscle twitch.

Original language	English (US)
Journal	American Journal of Physiology - Cell Physiology
Volume	274
Issue number	3 43-3
State	Published - Mar 1998

Keywords

Energetics
Metabolism
Nuclear magnetic resonance
Twitch

ASJC Scopus subject areas

Clinical Biochemistry
Cell Biology
Physiology
Physiology (medical)

Cite this

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title = "Metabolic fluctuation during a muscle contraction cycle",

abstract = "Gated 31P-nuclear magnetic resonance followed the metabolic fluctuation in rat gastrocnemius muscle during a contraction cycle. Within 16 ms after stimulation, the phosphocreatine (PCr) level drops 11.3% from its reference state. The PCr minimum corresponds closely to the time of maximum force contraction. P(i) increases stoichiometrically, while ATP remains constant. During a twitch, PCr hydrolysis produces 3.1 μmol ATP/g tissue, which is substantially higher than the reported 0.3 μmol ATP·twitch-·g tissue-1 derived from steady-state experiments. The results reveal that a substantial energy fluctuation accompanies a muscle twitch.",

keywords = "Energetics, Metabolism, Nuclear magnetic resonance, Twitch",

author = "Youngran Chung and Robert Sharman and Richard Carlsen and Unger, {Steven W.} and Douglas Larson and Thomas Jue",

year = "1998",

month = mar,

language = "English (US)",

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T1 - Metabolic fluctuation during a muscle contraction cycle

AU - Chung, Youngran

AU - Sharman, Robert

AU - Carlsen, Richard

AU - Unger, Steven W.

AU - Larson, Douglas

AU - Jue, Thomas

PY - 1998/3

Y1 - 1998/3

N2 - Gated 31P-nuclear magnetic resonance followed the metabolic fluctuation in rat gastrocnemius muscle during a contraction cycle. Within 16 ms after stimulation, the phosphocreatine (PCr) level drops 11.3% from its reference state. The PCr minimum corresponds closely to the time of maximum force contraction. P(i) increases stoichiometrically, while ATP remains constant. During a twitch, PCr hydrolysis produces 3.1 μmol ATP/g tissue, which is substantially higher than the reported 0.3 μmol ATP·twitch-·g tissue-1 derived from steady-state experiments. The results reveal that a substantial energy fluctuation accompanies a muscle twitch.

AB - Gated 31P-nuclear magnetic resonance followed the metabolic fluctuation in rat gastrocnemius muscle during a contraction cycle. Within 16 ms after stimulation, the phosphocreatine (PCr) level drops 11.3% from its reference state. The PCr minimum corresponds closely to the time of maximum force contraction. P(i) increases stoichiometrically, while ATP remains constant. During a twitch, PCr hydrolysis produces 3.1 μmol ATP/g tissue, which is substantially higher than the reported 0.3 μmol ATP·twitch-·g tissue-1 derived from steady-state experiments. The results reveal that a substantial energy fluctuation accompanies a muscle twitch.

KW - Energetics

KW - Metabolism

KW - Nuclear magnetic resonance

KW - Twitch

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SN - 1931-857X

IS - 3 43-3

ER -

Metabolic fluctuation during a muscle contraction cycle

Abstract

Keywords

ASJC Scopus subject areas

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