Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887

Beatrix Miu, Thomas P. Martin, Roland R. Roy, V. Oganov, E. Ilyinakakueva, J. F. Marini, J. J. Leger, Sue C. Bodine-Fowler, V. Reggie Edgerton

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The adaptation of a slow (soleus, Sol) and a fast (medial gastrocnemius, MG) skeletal muscle to space-flight was studied in five young male rats. The flight period was 12.5 days and the rats were killed approximately 48 h after returning to 1 g. Five other rats that were housed in cages similar to those used by the flight rats were maintained at 1 g for the same period of time to serve as ground-based controls. Fibers were classified as dark or light staining for myosin adenosine triphosphatase (ATPase). On the average, the fibers in the Sol of the flight rats atrophied twice as much as those in the MG. Further, the fibers located in the deep (close to the bone and having the highest percentage of light ATPase and high oxidative fibers in the muscle cross section) region of the MG atrophied more than the fibers located in the superficial (away from the bone and having the lowest percentage ol light ATPase and high oxidative fibers in the muscle cross-section) region of the muscle. Based on quantitative histochemical assays of single muscle fibers, succinate dehydrogenase (SDH) activity per unit volume was unchanged in fibers of the Sol and MG. However, in the Sol, but not the MG, the total amount of SDH activity in a 10-μm-thick section of a fiber decreased significantly in response to space-flight. Based on population distributions, it appears that the α-glycerophosphate dehydrogenase (GPD) activities were elevated in the dark ATPase fibers in the Sol, whereas the light fibers in the Sol and both fiber types in the MG did not appear to change. The ratio of GPD to SDH activities increased in the dark (but not light) fibers of the Sol and was unaffected in the MG. Immunohistochemical analyses indicate that approximately 40% of the fibers in the Sol of flight rats expressed a fast myosin heavy chain compared with 22% in control rats. Further, 31% of the fibers in the Sol of flight rats expressed both fast and slow myosin heavy chains compared with 8% in control rats. Immunohistochemical changes in the MG were minimal. These data suggest that the magnitude and direction of enzymatic activity and cell volume changes are dependent on the muscle, the region of the muscle, and the type of myosin expressed in the fibers. Further, the ability of fibers to maintain normal or even elevated activities per unit volume of some metabolic enzymes is remarkable considering the marked and rapid decrease in fiber volume.

Original languageEnglish (US)
Pages (from-to)64-72
Number of pages9
JournalFASEB Journal
Volume4
Issue number1
StatePublished - 1990

Fingerprint

Cosmos
space flight
Space Flight
muscle fibers
Muscle
Rats
Muscles
Fibers
rats
flight
adenosinetriphosphatase
Succinate Dehydrogenase
Light
Glycerolphosphate Dehydrogenase
Adenosine Triphosphatases
Myosin Heavy Chains
myosin heavy chains
Myosins
myosin
muscles

Keywords

  • Immunofluorescence
  • Muscle atrophy
  • Myosin ATPase
  • Myosin heavy chain
  • Quantitative histochemistry
  • Weightlessness

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Miu, B., Martin, T. P., Roy, R. R., Oganov, V., Ilyinakakueva, E., Marini, J. F., ... Edgerton, V. R. (1990). Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887. FASEB Journal, 4(1), 64-72.

Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887. / Miu, Beatrix; Martin, Thomas P.; Roy, Roland R.; Oganov, V.; Ilyinakakueva, E.; Marini, J. F.; Leger, J. J.; Bodine-Fowler, Sue C.; Edgerton, V. Reggie.

In: FASEB Journal, Vol. 4, No. 1, 1990, p. 64-72.

Research output: Contribution to journalArticle

Miu, B, Martin, TP, Roy, RR, Oganov, V, Ilyinakakueva, E, Marini, JF, Leger, JJ, Bodine-Fowler, SC & Edgerton, VR 1990, 'Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887', FASEB Journal, vol. 4, no. 1, pp. 64-72.
Miu B, Martin TP, Roy RR, Oganov V, Ilyinakakueva E, Marini JF et al. Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887. FASEB Journal. 1990;4(1):64-72.
Miu, Beatrix ; Martin, Thomas P. ; Roy, Roland R. ; Oganov, V. ; Ilyinakakueva, E. ; Marini, J. F. ; Leger, J. J. ; Bodine-Fowler, Sue C. ; Edgerton, V. Reggie. / Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887. In: FASEB Journal. 1990 ; Vol. 4, No. 1. pp. 64-72.
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AU - Ilyinakakueva, E.

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