Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model

Brent E. Wilkens, Darryl L. Millis, Gregory B. Daniel, Linda Munson, Kanaiyala R. Patel, Frances C. Buonomo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective - To investigate the effect of recombinant canine somatotropin (STH) on the metabolic and histologic aspects of bone healing in dogs, using an unstable ostectomy gap model. Animals - 8 mature dogs. Procedure - A 3-mm ostectomy of the mid portion of the radius was performed in all dogs. Implants designed to release STH at a rate of 4 mg/d were placed SC in 4 dogs (treated group [STHG]), and another 4 dogs received no implants (control group [CG]). Serum concentrations of STH, insulin-like growth factor I, and osteocalcin were determined before surgery, and weekly for 8 weeks. Scintigraphic evaluation of the ostectomy sites was performed before surgery, and at weeks 2, 4, 6, and 8 after surgery. Histologic evaluation of the ostectomy sites was performed at the conclusion of the study at week 8. Results - Significant (P < 0.05) increases in serum STH, insulin-like growth factor I, and osteocalcin concentrations were observed in dogs of the STHG during the 8-week study period. Scintigraphic activity of the ostectomy sites was increased in dogs of both groups, but dogs of the STHG had significantly (P < 0.05) greater activity, compared with dogs of the CG. Coalescence of nuclear activity across the ostectomy site was observed in dogs of the STHG, whereas dogs of the CG maintained 2 distinct areas of metabolic activity. Histologically, dogs of the STHG had bridging calluses with areas of endochondral ossification and ongoing osteogenic activity, whereas dogs of the CG had nonossified fibrocartilage typical of nonunion fractures. Conclusion - Using the ostectomy gap model, recombinant canine STH enhanced the metabolic and histologic aspects of bone healing in dogs.

Original languageEnglish (US)
Pages (from-to)1395-1401
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume57
Issue number9
StatePublished - 1996
Externally publishedYes

Fingerprint

somatotropin
Growth Hormone
Canidae
bones
Dogs
Bone and Bones
dogs
Control Groups
Osteocalcin
osteocalcin
surgery
Insulin-Like Growth Factor I
insulin-like growth factor I
Fibrocartilage
Bony Callus
radius (bone)
Serum
Osteogenesis
bone formation
callus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Wilkens, B. E., Millis, D. L., Daniel, G. B., Munson, L., Patel, K. R., & Buonomo, F. C. (1996). Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model. American Journal of Veterinary Research, 57(9), 1395-1401.

Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model. / Wilkens, Brent E.; Millis, Darryl L.; Daniel, Gregory B.; Munson, Linda; Patel, Kanaiyala R.; Buonomo, Frances C.

In: American Journal of Veterinary Research, Vol. 57, No. 9, 1996, p. 1395-1401.

Research output: Contribution to journalArticle

Wilkens, BE, Millis, DL, Daniel, GB, Munson, L, Patel, KR & Buonomo, FC 1996, 'Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model', American Journal of Veterinary Research, vol. 57, no. 9, pp. 1395-1401.
Wilkens, Brent E. ; Millis, Darryl L. ; Daniel, Gregory B. ; Munson, Linda ; Patel, Kanaiyala R. ; Buonomo, Frances C. / Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model. In: American Journal of Veterinary Research. 1996 ; Vol. 57, No. 9. pp. 1395-1401.
@article{2010c7bfabb740aeb82c6d0c11361bd5,
title = "Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model",
abstract = "Objective - To investigate the effect of recombinant canine somatotropin (STH) on the metabolic and histologic aspects of bone healing in dogs, using an unstable ostectomy gap model. Animals - 8 mature dogs. Procedure - A 3-mm ostectomy of the mid portion of the radius was performed in all dogs. Implants designed to release STH at a rate of 4 mg/d were placed SC in 4 dogs (treated group [STHG]), and another 4 dogs received no implants (control group [CG]). Serum concentrations of STH, insulin-like growth factor I, and osteocalcin were determined before surgery, and weekly for 8 weeks. Scintigraphic evaluation of the ostectomy sites was performed before surgery, and at weeks 2, 4, 6, and 8 after surgery. Histologic evaluation of the ostectomy sites was performed at the conclusion of the study at week 8. Results - Significant (P < 0.05) increases in serum STH, insulin-like growth factor I, and osteocalcin concentrations were observed in dogs of the STHG during the 8-week study period. Scintigraphic activity of the ostectomy sites was increased in dogs of both groups, but dogs of the STHG had significantly (P < 0.05) greater activity, compared with dogs of the CG. Coalescence of nuclear activity across the ostectomy site was observed in dogs of the STHG, whereas dogs of the CG maintained 2 distinct areas of metabolic activity. Histologically, dogs of the STHG had bridging calluses with areas of endochondral ossification and ongoing osteogenic activity, whereas dogs of the CG had nonossified fibrocartilage typical of nonunion fractures. Conclusion - Using the ostectomy gap model, recombinant canine STH enhanced the metabolic and histologic aspects of bone healing in dogs.",
author = "Wilkens, {Brent E.} and Millis, {Darryl L.} and Daniel, {Gregory B.} and Linda Munson and Patel, {Kanaiyala R.} and Buonomo, {Frances C.}",
year = "1996",
language = "English (US)",
volume = "57",
pages = "1395--1401",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "9",

}

TY - JOUR

T1 - Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model

AU - Wilkens, Brent E.

AU - Millis, Darryl L.

AU - Daniel, Gregory B.

AU - Munson, Linda

AU - Patel, Kanaiyala R.

AU - Buonomo, Frances C.

PY - 1996

Y1 - 1996

N2 - Objective - To investigate the effect of recombinant canine somatotropin (STH) on the metabolic and histologic aspects of bone healing in dogs, using an unstable ostectomy gap model. Animals - 8 mature dogs. Procedure - A 3-mm ostectomy of the mid portion of the radius was performed in all dogs. Implants designed to release STH at a rate of 4 mg/d were placed SC in 4 dogs (treated group [STHG]), and another 4 dogs received no implants (control group [CG]). Serum concentrations of STH, insulin-like growth factor I, and osteocalcin were determined before surgery, and weekly for 8 weeks. Scintigraphic evaluation of the ostectomy sites was performed before surgery, and at weeks 2, 4, 6, and 8 after surgery. Histologic evaluation of the ostectomy sites was performed at the conclusion of the study at week 8. Results - Significant (P < 0.05) increases in serum STH, insulin-like growth factor I, and osteocalcin concentrations were observed in dogs of the STHG during the 8-week study period. Scintigraphic activity of the ostectomy sites was increased in dogs of both groups, but dogs of the STHG had significantly (P < 0.05) greater activity, compared with dogs of the CG. Coalescence of nuclear activity across the ostectomy site was observed in dogs of the STHG, whereas dogs of the CG maintained 2 distinct areas of metabolic activity. Histologically, dogs of the STHG had bridging calluses with areas of endochondral ossification and ongoing osteogenic activity, whereas dogs of the CG had nonossified fibrocartilage typical of nonunion fractures. Conclusion - Using the ostectomy gap model, recombinant canine STH enhanced the metabolic and histologic aspects of bone healing in dogs.

AB - Objective - To investigate the effect of recombinant canine somatotropin (STH) on the metabolic and histologic aspects of bone healing in dogs, using an unstable ostectomy gap model. Animals - 8 mature dogs. Procedure - A 3-mm ostectomy of the mid portion of the radius was performed in all dogs. Implants designed to release STH at a rate of 4 mg/d were placed SC in 4 dogs (treated group [STHG]), and another 4 dogs received no implants (control group [CG]). Serum concentrations of STH, insulin-like growth factor I, and osteocalcin were determined before surgery, and weekly for 8 weeks. Scintigraphic evaluation of the ostectomy sites was performed before surgery, and at weeks 2, 4, 6, and 8 after surgery. Histologic evaluation of the ostectomy sites was performed at the conclusion of the study at week 8. Results - Significant (P < 0.05) increases in serum STH, insulin-like growth factor I, and osteocalcin concentrations were observed in dogs of the STHG during the 8-week study period. Scintigraphic activity of the ostectomy sites was increased in dogs of both groups, but dogs of the STHG had significantly (P < 0.05) greater activity, compared with dogs of the CG. Coalescence of nuclear activity across the ostectomy site was observed in dogs of the STHG, whereas dogs of the CG maintained 2 distinct areas of metabolic activity. Histologically, dogs of the STHG had bridging calluses with areas of endochondral ossification and ongoing osteogenic activity, whereas dogs of the CG had nonossified fibrocartilage typical of nonunion fractures. Conclusion - Using the ostectomy gap model, recombinant canine STH enhanced the metabolic and histologic aspects of bone healing in dogs.

UR - http://www.scopus.com/inward/record.url?scp=0030228442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030228442&partnerID=8YFLogxK

M3 - Article

C2 - 8874740

AN - SCOPUS:0030228442

VL - 57

SP - 1395

EP - 1401

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 9

ER -