Mesenchymal cells stimulate capillary morphogenesis via distinct proteolytic mechanisms

Cyrus M. Ghajar, Suraj Kachgal, Ekaterina Kniazeva, Hidetoshi Mori, Sylvain V. Costes, Steven George, Andrew J. Putnam

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

During angiogenesis, endothelial cells (ECs) degrade their surrounding extracellular matrix (ECM) to facilitate invasion. How interactions between ECs and other cells within their microenvironment facilitate this process is only partially understood. We have utilized a tractable 3D co-culture model to investigate the proteolytic mechanisms by which pre-committed or more highly committed mesenchymal cells stimulate capillary formation. On their own, ECs invade their surrounding matrix, but do not form capillaries. However, in the presence of either mesenchymal stem cells (MSCs) or fibroblasts, ECs form polarized, tubular structures that are intimately associated with mesenchymal cells. Further, ECs up-regulate gene expression of several extracellular proteases upon co-culture with either mesenchymal cell type. The administration of both broad spectrum and specific protease inhibitors demonstrated that MSC-stimulated capillary formation relied solely on membrane-type matrix metalloproteinases (MT-MMPs) while fibroblast-mediated sprouting proceeded independent of MMP inhibition unless the plasminogen activator/plasmin axis was inhibited in concert. While other studies have established a role for the ECM itself in dictating proteolysis and matrix degradation during capillary morphogenesis, the present study illustrates that heterotypic cellular interactions within the microenvironment can direct the proteolytic mechanisms required for capillary formation.

Original languageEnglish (US)
Pages (from-to)813-825
Number of pages13
JournalExperimental Cell Research
Volume316
Issue number5
DOIs
StatePublished - Mar 10 2010

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Morphogenesis
Endothelial Cells
Coculture Techniques
Mesenchymal Stromal Cells
Extracellular Matrix
Membrane-Associated Matrix Metalloproteinases
Fibroblasts
Plasminogen Activators
Fibrinolysin
Protease Inhibitors
Matrix Metalloproteinases
Proteolysis
Peptide Hydrolases
Up-Regulation
Gene Expression

Keywords

  • Angiogenesis
  • Capillary morphogenesis
  • Fibroblasts
  • Matrix metalloproteinases
  • Mesenchymal stem cells
  • Microenvironment

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mesenchymal cells stimulate capillary morphogenesis via distinct proteolytic mechanisms. / Ghajar, Cyrus M.; Kachgal, Suraj; Kniazeva, Ekaterina; Mori, Hidetoshi; Costes, Sylvain V.; George, Steven; Putnam, Andrew J.

In: Experimental Cell Research, Vol. 316, No. 5, 10.03.2010, p. 813-825.

Research output: Contribution to journalArticle

Ghajar, Cyrus M. ; Kachgal, Suraj ; Kniazeva, Ekaterina ; Mori, Hidetoshi ; Costes, Sylvain V. ; George, Steven ; Putnam, Andrew J. / Mesenchymal cells stimulate capillary morphogenesis via distinct proteolytic mechanisms. In: Experimental Cell Research. 2010 ; Vol. 316, No. 5. pp. 813-825.
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