Membranous nephropathy, interstitial nephritis, and Fanconi syndrome - Glomerular antigen

Sudesh P Makker, Richard Widstrom, Jianmin Huang

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We characterized the glomerular antigen of membranous nephropathy (MN) in a child with the triad of MN, proximal renal tubular basement membrane autoantibody (TBMAb)-associated interstitial nephritis (ITN), and Fanconi syndrome. Granular staining was demonstrated for human gp600 in the vicinity of immune deposits of MN along glomerular capillary loops, using a monospecific polyclonal antibody to human gp600 by indirect immunofluorescence. However, no staining was observed in the MN deposits for receptor-associated protein. Membranous nephropathy preceded the development of TBMAbs, ITN, and Fanconi syndrome by 1 year, showing that the MN lesion does not result from the initial immunological injury to the tubulointerstitium, as postulated earlier. We confirmed the reactivity of TBMAbs with the recently described rabbit 58-kilodalton (kDa) tubular basement membrane antigen (TBMAg). However, this is the first report to show reactivity of these antibodies with the human 58-kDa protein. Also we found that TBMAg is comprised of a single protein band of 58 kDa, unlike the previously described combination of two protein bands (58 kDa and 175 kDa). In this patient, following prednisone treatment, the TBMAbs became undetectable, and the nephrotic syndrome and Fanconi syndrome resolved, thus suggesting a causal role of TBMAbs in the pathogenesis of Fanconi syndrome. We postulate that in this rare disorder, renal lesions result from an autoimmune response to the 58-kDa TBMAg and possibly to gp600, and that the predisposition to autoimmunity is genetically linked to the HLA B7 serotype.

Original languageEnglish (US)
Pages (from-to)7-13
Number of pages7
JournalPediatric Nephrology
Volume10
Issue number1
DOIs
StatePublished - Feb 1996

Keywords

  • Fanconi syndrome
  • gp 330
  • gp 600
  • Heymann antigen
  • Heymann nephritis
  • Membranous nephropathy
  • Tubular basement membrane autoantibodies

ASJC Scopus subject areas

  • Nephrology
  • Pediatrics, Perinatology, and Child Health

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