Membrane type 4 matrix metalloproteinase (MT4-MMP, MMP-17) is a glycosylphosphatidylinositol-anchored proteinase

Yoshifumi Itoh, Masahiro Kajita, Hiroaki Kinoh, Hidetoshi Mori, Akiko Okada, Motoharu Seiki

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Among the five membrane-type matrix metalloproteinases (MT-MMPs), MT1-, MT2-, MT3-, and MT5-MMPs have about a 20-amino acid cytoplasmic tail following the transmembrane domain. In contrast, a putative transmembrane domain of MT4-MMP locates at the very C-terminal end, and the expected cytoplasmic tail is very short or nonexistent. Such sequences often act as a glycosylphosphatidylinositol (GPI) anchoring signal rather than as a transmembrane domain. We thus examined the possibility that MT4-MMP is a GPI- anchored proteinase. Our results showed that [3H]ethanolamine, which can be incorporated into the GPI unit, specifically labeled the MT4-MMP C-terminal end in a sequence-dependent manner. In addition, phosphatidylinositol- specific phospholipase C treatment released the MT4-MMP from the surface of transfected cells. These results indicate that MT4-MMP is the first GPI- anchored proteinase in the MMP family. During cultivation of the transfected cells, MT4-MMP appeared to be shed from the cell surface by the action of an endogenous metalloproteinase. GPI anchoring of MT4-MMP on the cell surface indicates a unique biological function and character for this proteinase.

Original languageEnglish (US)
Pages (from-to)34260-34266
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number48
DOIs
StatePublished - Nov 26 1999
Externally publishedYes

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Matrix Metalloproteinase 17
Glycosylphosphatidylinositols
Matrix Metalloproteinases
Peptide Hydrolases
Matrix Metalloproteinase 16
Membrane-Associated Matrix Metalloproteinases
Phosphoinositide Phospholipase C
Ethanolamine
Metalloproteases
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Membrane type 4 matrix metalloproteinase (MT4-MMP, MMP-17) is a glycosylphosphatidylinositol-anchored proteinase. / Itoh, Yoshifumi; Kajita, Masahiro; Kinoh, Hiroaki; Mori, Hidetoshi; Okada, Akiko; Seiki, Motoharu.

In: Journal of Biological Chemistry, Vol. 274, No. 48, 26.11.1999, p. 34260-34266.

Research output: Contribution to journalArticle

Itoh, Yoshifumi ; Kajita, Masahiro ; Kinoh, Hiroaki ; Mori, Hidetoshi ; Okada, Akiko ; Seiki, Motoharu. / Membrane type 4 matrix metalloproteinase (MT4-MMP, MMP-17) is a glycosylphosphatidylinositol-anchored proteinase. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 48. pp. 34260-34266.
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