Membrane tension, myosin force, and actin turnover maintain actin treadmill in the nerve growth cone

Erin M. Craig, David Van Goor, Paul Forscher, Alex Mogilner

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A growth cone is a motile structure at the tips of axons that is driven by the actin network and guides axon extension. Low actin adhesion to the substrate creates a stationary actin treadmill that allows leading-edge protrusion when adhesion increases in response to guidance cues. We use experimental measurements in the Aplysia bag growth cone to develop and constrain a simple mechanical model of the actin treadmill. We show that actin retrograde flow is primarily generated by myosin contractile forces, but when myosin is inhibited, leading-edge membrane tension increases and drives the flow. By comparing predictions of the model with previous experimental measurements, we demonstrate that lamellipodial and filopodial filament breaking contribute equally to the resistance to the flow. The fully constrained model clarifies the role of actin turnover in the mechanical balance driving the actin treadmill and reproduces the recent experimental observation that inhibition of actin depolymerization causes retrograde flow to slow exponentially with time. We estimate forces in the actin treadmill, and we demonstrate that measured G-actin distributions are consistent with the existence of a forward-directed fluid flow that transports G-actin to the leading edge.

Original languageEnglish (US)
Pages (from-to)1503-1513
Number of pages11
JournalBiophysical Journal
Volume102
Issue number7
DOIs
StatePublished - Apr 4 2012

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Growth Cones
Myosins
Actins
Membranes
Axons
Aplysia
Cues

ASJC Scopus subject areas

  • Biophysics

Cite this

Membrane tension, myosin force, and actin turnover maintain actin treadmill in the nerve growth cone. / Craig, Erin M.; Van Goor, David; Forscher, Paul; Mogilner, Alex.

In: Biophysical Journal, Vol. 102, No. 7, 04.04.2012, p. 1503-1513.

Research output: Contribution to journalArticle

Craig, Erin M. ; Van Goor, David ; Forscher, Paul ; Mogilner, Alex. / Membrane tension, myosin force, and actin turnover maintain actin treadmill in the nerve growth cone. In: Biophysical Journal. 2012 ; Vol. 102, No. 7. pp. 1503-1513.
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