Membrane phase transition of intact human platelets

Correlation with cold-induced activation

Fern Tablin, Ann E. Oliver, Naomi J. Walker, Lois M. Crowe, John H. Crowe

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Using Fourier transform infrared spectroscopy (FTIR), we have determined the phase transition temperature (T(m)) of lipids in intact human platelets and have shown that it occurs between 15 and 18°C, the temperature at which cold activation of platelets has previously been reported (Zucker and Borrelli, 1954, Blood, 28:602-608; White and Krivit, 1967, Blood, 30:625-635). The temperature at which the platelets pass through T(m) is highly correlated with initial platelet shape change. However, shape change continues after the cells have passed through the phase transition. Cold-induced activation has previously prevented long-term storage of platelets at 4°C. Antifreeze glycoproteins (AFGPs) isolated from polar fishes previously have been used to prevent ice crystal growth during freezing of tissues as well as leakage of solutes from liposomes as they were chilled through their T(m). We sought to determine if these AFGPs were able to stabilize platelets for long-term storage at 4°C. Incubating platelets with antifreeze glycoproteins during long-term storage and rapid rewarming to 37°C abrogated granule secretion associated with cold activation in a dose-dependent manner. This work suggests that AFGPs may be a possible solute for use in long-term low temperature storage of platelets.

Original languageEnglish (US)
Pages (from-to)305-313
Number of pages9
JournalJournal of Cellular Physiology
Volume168
Issue number2
DOIs
StatePublished - Aug 1996

Fingerprint

Phase Transition
Platelets
Antifreeze Proteins
Blood Platelets
Phase transitions
Chemical activation
Membranes
Temperature
Blood
Rewarming
Transition Temperature
Platelet Activation
Ice
Fourier Transform Infrared Spectroscopy
Crystallization
Liposomes
Freezing
Fishes
Fish
Lipids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Membrane phase transition of intact human platelets : Correlation with cold-induced activation. / Tablin, Fern; Oliver, Ann E.; Walker, Naomi J.; Crowe, Lois M.; Crowe, John H.

In: Journal of Cellular Physiology, Vol. 168, No. 2, 08.1996, p. 305-313.

Research output: Contribution to journalArticle

Tablin, Fern ; Oliver, Ann E. ; Walker, Naomi J. ; Crowe, Lois M. ; Crowe, John H. / Membrane phase transition of intact human platelets : Correlation with cold-induced activation. In: Journal of Cellular Physiology. 1996 ; Vol. 168, No. 2. pp. 305-313.
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