Abstract
Using Fourier transform infrared spectroscopy (FTIR), we have determined the phase transition temperature (T(m)) of lipids in intact human platelets and have shown that it occurs between 15 and 18°C, the temperature at which cold activation of platelets has previously been reported (Zucker and Borrelli, 1954, Blood, 28:602-608; White and Krivit, 1967, Blood, 30:625-635). The temperature at which the platelets pass through T(m) is highly correlated with initial platelet shape change. However, shape change continues after the cells have passed through the phase transition. Cold-induced activation has previously prevented long-term storage of platelets at 4°C. Antifreeze glycoproteins (AFGPs) isolated from polar fishes previously have been used to prevent ice crystal growth during freezing of tissues as well as leakage of solutes from liposomes as they were chilled through their T(m). We sought to determine if these AFGPs were able to stabilize platelets for long-term storage at 4°C. Incubating platelets with antifreeze glycoproteins during long-term storage and rapid rewarming to 37°C abrogated granule secretion associated with cold activation in a dose-dependent manner. This work suggests that AFGPs may be a possible solute for use in long-term low temperature storage of platelets.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 305-313 |
| Number of pages | 9 |
| Journal | Journal of Cellular Physiology |
| Volume | 168 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1996 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
Membrane phase transition of intact human platelets : Correlation with cold-induced activation. / Tablin, Fern; Oliver, Ann E.; Walker, Naomi J.; Crowe, Lois M.; Crowe, John H.
In: Journal of Cellular Physiology, Vol. 168, No. 2, 08.1996, p. 305-313.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Membrane phase transition of intact human platelets
T2 - Correlation with cold-induced activation
AU - Tablin, Fern
AU - Oliver, Ann E.
AU - Walker, Naomi J.
AU - Crowe, Lois M.
AU - Crowe, John H.
PY - 1996/8
Y1 - 1996/8
N2 - Using Fourier transform infrared spectroscopy (FTIR), we have determined the phase transition temperature (T(m)) of lipids in intact human platelets and have shown that it occurs between 15 and 18°C, the temperature at which cold activation of platelets has previously been reported (Zucker and Borrelli, 1954, Blood, 28:602-608; White and Krivit, 1967, Blood, 30:625-635). The temperature at which the platelets pass through T(m) is highly correlated with initial platelet shape change. However, shape change continues after the cells have passed through the phase transition. Cold-induced activation has previously prevented long-term storage of platelets at 4°C. Antifreeze glycoproteins (AFGPs) isolated from polar fishes previously have been used to prevent ice crystal growth during freezing of tissues as well as leakage of solutes from liposomes as they were chilled through their T(m). We sought to determine if these AFGPs were able to stabilize platelets for long-term storage at 4°C. Incubating platelets with antifreeze glycoproteins during long-term storage and rapid rewarming to 37°C abrogated granule secretion associated with cold activation in a dose-dependent manner. This work suggests that AFGPs may be a possible solute for use in long-term low temperature storage of platelets.
AB - Using Fourier transform infrared spectroscopy (FTIR), we have determined the phase transition temperature (T(m)) of lipids in intact human platelets and have shown that it occurs between 15 and 18°C, the temperature at which cold activation of platelets has previously been reported (Zucker and Borrelli, 1954, Blood, 28:602-608; White and Krivit, 1967, Blood, 30:625-635). The temperature at which the platelets pass through T(m) is highly correlated with initial platelet shape change. However, shape change continues after the cells have passed through the phase transition. Cold-induced activation has previously prevented long-term storage of platelets at 4°C. Antifreeze glycoproteins (AFGPs) isolated from polar fishes previously have been used to prevent ice crystal growth during freezing of tissues as well as leakage of solutes from liposomes as they were chilled through their T(m). We sought to determine if these AFGPs were able to stabilize platelets for long-term storage at 4°C. Incubating platelets with antifreeze glycoproteins during long-term storage and rapid rewarming to 37°C abrogated granule secretion associated with cold activation in a dose-dependent manner. This work suggests that AFGPs may be a possible solute for use in long-term low temperature storage of platelets.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029737003&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4652(199608)168:2<305::AID-JCP9>3.0.CO;2-T
DO - 10.1002/(SICI)1097-4652(199608)168:2<305::AID-JCP9>3.0.CO;2-T
M3 - Article
C2 - 8707866
AN - SCOPUS:0029737003
VL - 168
SP - 305
EP - 313
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 2
ER -