Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation

Diane Dayoung Park, Gege Xu, Maurice Wong, Chatchai Phoomak, Mingqi Liu, Nathan E. Haigh, Sopit Wongkham, Pengyuan Yang, Emanual Michael Maverakis, Carlito B Lebrilla

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface. However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties. Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids. To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain. Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.

Original languageEnglish (US)
Pages (from-to)6271-6285
Number of pages15
JournalChemical Science
Volume9
Issue number29
DOIs
StatePublished - Jan 1 2018

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Membranes
Sialic Acids
Glycosylation
Glycosphingolipids
Glycoconjugates
Sugars
Polysaccharides
Imaging techniques
Kinetics
Glycomics
Epithelial Cells

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Park, D. D., Xu, G., Wong, M., Phoomak, C., Liu, M., Haigh, N. E., ... Lebrilla, C. B. (2018). Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation. Chemical Science, 9(29), 6271-6285. https://doi.org/10.1039/c8sc01875h

Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation. / Park, Diane Dayoung; Xu, Gege; Wong, Maurice; Phoomak, Chatchai; Liu, Mingqi; Haigh, Nathan E.; Wongkham, Sopit; Yang, Pengyuan; Maverakis, Emanual Michael; Lebrilla, Carlito B.

In: Chemical Science, Vol. 9, No. 29, 01.01.2018, p. 6271-6285.

Research output: Contribution to journalArticle

Park, DD, Xu, G, Wong, M, Phoomak, C, Liu, M, Haigh, NE, Wongkham, S, Yang, P, Maverakis, EM & Lebrilla, CB 2018, 'Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation', Chemical Science, vol. 9, no. 29, pp. 6271-6285. https://doi.org/10.1039/c8sc01875h
Park, Diane Dayoung ; Xu, Gege ; Wong, Maurice ; Phoomak, Chatchai ; Liu, Mingqi ; Haigh, Nathan E. ; Wongkham, Sopit ; Yang, Pengyuan ; Maverakis, Emanual Michael ; Lebrilla, Carlito B. / Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation. In: Chemical Science. 2018 ; Vol. 9, No. 29. pp. 6271-6285.
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