Membrane curvature induction and tubulation are common features of synucleins and apolipoproteins

Jobin Varkey, Jose Mario Isas, Naoko Mizuno, Martin Borch Jensen, Vikram Kjøller Bhatia, Christine C. Jao, Jitka Petrlova, John C Voss, Dimitrios G. Stamou, Alasdair C. Steven, Ralf Langen

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Synucleins and apolipoproteins have been implicated in a number of membrane and lipid trafficking events. Lipid interaction for both types of proteins is mediated by 11 amino acid repeats that form amphipathic helices. This similarity suggests that synucleins and apolipoproteins might have comparable effects on lipid membranes, but this has not been shown directly. Here, we find that α-synuclein, β-synuclein, and apolipoprotein A-1 have the conserved functional ability to induce membrane curvature and to convert large vesicles into highly curved membrane tubules and vesicles. The resulting structures are morphologically similar to those generated by amphiphysin, a curvature-inducing protein involved in endocytosis. Unlike amphiphysin, however, synucleins and apolipoproteins do not require any scaffolding domains and curvature induction is mediated by the membrane insertion and wedging of amphipathic helices alone. Moreover, we frequently observed that α-synuclein caused membrane structures that had the appearance of nascent budding vesicles. The ability to function as a minimal machinery for vesicle budding agrees well with recent findings that α-synuclein plays a role in vesicle trafficking and enhances endocytosis. Induction of membrane curvature must be under strict regulation in vivo; however, as we find it can also cause disruption of membrane integrity. Because the degree of membrane curvature induction depends on the concerted action of multiple proteins, controlling the local protein density of tubulating proteins may be important. How cellular safeguarding mechanisms prevent such potentially toxic events and whether they go awry in disease remains to be determined.

Original languageEnglish (US)
Pages (from-to)32486-32493
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number42
DOIs
StatePublished - Oct 15 2010

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Synucleins
Apolipoproteins
Membranes
Membrane Lipids
Endocytosis
Proteins
Membrane structures
Poisons
Apolipoprotein A-I
Machinery
Lipids
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Varkey, J., Isas, J. M., Mizuno, N., Jensen, M. B., Bhatia, V. K., Jao, C. C., ... Langen, R. (2010). Membrane curvature induction and tubulation are common features of synucleins and apolipoproteins. Journal of Biological Chemistry, 285(42), 32486-32493. https://doi.org/10.1074/jbc.M110.139576

Membrane curvature induction and tubulation are common features of synucleins and apolipoproteins. / Varkey, Jobin; Isas, Jose Mario; Mizuno, Naoko; Jensen, Martin Borch; Bhatia, Vikram Kjøller; Jao, Christine C.; Petrlova, Jitka; Voss, John C; Stamou, Dimitrios G.; Steven, Alasdair C.; Langen, Ralf.

In: Journal of Biological Chemistry, Vol. 285, No. 42, 15.10.2010, p. 32486-32493.

Research output: Contribution to journalArticle

Varkey, J, Isas, JM, Mizuno, N, Jensen, MB, Bhatia, VK, Jao, CC, Petrlova, J, Voss, JC, Stamou, DG, Steven, AC & Langen, R 2010, 'Membrane curvature induction and tubulation are common features of synucleins and apolipoproteins', Journal of Biological Chemistry, vol. 285, no. 42, pp. 32486-32493. https://doi.org/10.1074/jbc.M110.139576
Varkey, Jobin ; Isas, Jose Mario ; Mizuno, Naoko ; Jensen, Martin Borch ; Bhatia, Vikram Kjøller ; Jao, Christine C. ; Petrlova, Jitka ; Voss, John C ; Stamou, Dimitrios G. ; Steven, Alasdair C. ; Langen, Ralf. / Membrane curvature induction and tubulation are common features of synucleins and apolipoproteins. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 42. pp. 32486-32493.
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