Membrane composition can influence the rate of Al3+-mediated lipid oxidation: Effect of galactolipids

S. V. Verstraeten, Carl L Keen, M. S. Golub, P. I. Oteiza

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39 Scopus citations

Abstract

In the first part of the present study we investigated the effects of pre-natal and early postnatal exposure of mice to high levels of dietary Al3+ on myelin lipid composition and lipid oxidation. We found: (1) a significantly higher (104%; P < 0.01) content of brain myelin galactolipids in the high-Al3+ group than in controls, and, (2) a significant correlation (r2 = 0.70; P < 0.01) between the concentration of myelin galactolipids and TBARS (2-thiobarbituric acid-reactive substances) content, a parameter of lipid oxidation, Based on these results, we evaluated in an in vitro model (liposomes) whether galactolipids could affect the capacity of Al3+ to stimulate Fe2+-initiated lipid oxidation, and whether this effect could be due to the promotion of changes in membrane physical properties (membrane phase separation and rigidification). The presence of galactolipids (10-40 mol%) in the liposomes caused a concentration-dependent increase in the stimulatory effect of Al3+ on Fe2+-induced TBARS production, and on the ability of Al3+ to induce phase separation and membrane rigidification. The capacity of Al3+ (10-100 μM) to induce lateral phase separation in liposomes composed of phosphatidylcholine/phosphatidylserine/galactolipid (36:24:40, molar ratio) was correlated significantly (r2 = 0.99; P < 0.001) with the stimulatory action of Al3+ on Fe2+-induced TBARS production. We propose that the high content of galactolipids found in myelin from Al3+-intoxicated mice could favour Al3+-induced changes in membrane physical properties, with the subsequent acceleration of lipid oxidation rates.

Original languageEnglish (US)
Pages (from-to)833-838
Number of pages6
JournalBiochemical Journal
Volume333
Issue number3
StatePublished - Aug 1 1998

ASJC Scopus subject areas

  • Biochemistry

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    Verstraeten, S. V., Keen, C. L., Golub, M. S., & Oteiza, P. I. (1998). Membrane composition can influence the rate of Al3+-mediated lipid oxidation: Effect of galactolipids. Biochemical Journal, 333(3), 833-838.