Membrane association of the Tnp and Inh proteins of IS50(R)

A. DeLong, Michael Syvanen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Using a radioimmunoassay for the IS50(R) proteins Tnp and Inh, we found that both proteins were present primarily in the cytoplasm, but 3 to 11% of Tnp and 3 to 5% of Inh were found in association with the inner membrane. The fractions of total Tnp and Inh that became membrane bound were unaffected by the amount of Tnp and Inh synthesized in whole cells, provided that the ratio of total Tnp to total Inh was not changed. In addition, Inh was not found in the membrane fraction in Tnp- IS50(R) mutants, indicating that Tnp is required for Inh localization.

Original languageEnglish (US)
Pages (from-to)5516-5519
Number of pages4
JournalJournal of Bacteriology
Volume172
Issue number9
StatePublished - 1990

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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