Melittin-stimulated arachidonic acid metabolism by cultured malignant human epidermal keratinocytes

Robert H. Rice, Lawrence Levine

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Upon melittin stimulation, cultured SCC-13 keratinocytes release prostaglandins E2, F, 6-keto-F, thromboxane B2, leukotriene B4, and 6-sulfido-peptide-containing leukotrienes (SRS) into serum free medium. Release of prostaglandins E2, F, and SRS, normalized to cell protein, is 3- to 10-fold higher from rapidly growing than confluent cultures. Cells growing with hydrocortisone in the medium produce approximately twice the level of the cyclooxygenase-mediated metabolites PGE2 and PGF as those without hydrocortisone, but similar levels of the lipoxygenase-mediated metabolite SRS. The results demonstrate the potential utility of squamous carcinoma lines for investigating biochemical pathways of arachidonic acid metabolism in keratinocytes.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Oct 15 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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