Melanocytic naevi with globular and reticular dermoscopic patterns display distinct BRAF V600E expression profiles and histopathological patterns

M. A. Marchetti, Maija Ht Kiuru, K. J. Busam, A. A. Marghoob, A. Scope, S. W. Dusza, M. A. Cordova, M. Fonseca, X. Wu, A. C. Halpern

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Results BRAF V600E expression was detected in 11 of 12 globular naevi vs. four of 13 reticular naevi (91·7% vs. 30·1%, P = 0·004). A predominantly dermal growth pattern (P < 0·001) and the presence of large junctional nests (P = 0·017) were each associated with a globular dermoscopic pattern. The presence of either a predominantly dermal growth pattern or large junctional nests was found in 13 of 15 naevi positive for BRAF V600E and in two of 10 naevi negative for BRAF V600E (86·7% vs. 20%, P = 0·002).

Conclusions The frequency of BRAF V600E mutations differs in naevi distinguished by unique dermoscopic structures and microanatomical growth patterns. Globular naevi, which most often histologically correspond to a predominantly dermal growth pattern and/or the presence of large junctional nests, are significantly more likely to express BRAF V600E than reticular naevi. These preliminary results require validation, but may directly inform future studies of naevogenesis and melanoma genesis. What's already known about this topic? Activating BRAF V600E mutations have been detected in a majority of melanocytic naevi. What does this study add? Dermoscopically defined globular naevi, which are most often composed of large melanocytic nests and a predominantly dermal growth pattern, are at least three times more likely to express BRAF V600E than dermoscopically defined reticular naevi.

Methods We retrospectively identified histologically proven melanocytic naevi with banal reticular or globular dermoscopic patterns and evaluated BRAF V600E expression using immunohistochemistry.

Summary Background BRAF (v-raf murine sarcoma viral oncogene homologue B) V600E mutations have been detected with high frequency in melanocytic naevi. Few studies have stratified analyses by naevus dermoscopic pattern.

Objectives To determine the frequency of BRAF V600E expression and histopathological pattern in acquired melanocytic naevi distinguished by a globular vs. reticular dermoscopic pattern.

Original languageEnglish (US)
Pages (from-to)1060-1065
Number of pages6
JournalBritish Journal of Dermatology
Volume171
Issue number5
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

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Pigmented Nevus
Nevus
Growth
Skin
Mutation
Oncogenes
Sarcoma
Melanoma
Immunohistochemistry

ASJC Scopus subject areas

  • Dermatology

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Melanocytic naevi with globular and reticular dermoscopic patterns display distinct BRAF V600E expression profiles and histopathological patterns. / Marchetti, M. A.; Kiuru, Maija Ht; Busam, K. J.; Marghoob, A. A.; Scope, A.; Dusza, S. W.; Cordova, M. A.; Fonseca, M.; Wu, X.; Halpern, A. C.

In: British Journal of Dermatology, Vol. 171, No. 5, 01.11.2014, p. 1060-1065.

Research output: Contribution to journalArticle

Marchetti, MA, Kiuru, MH, Busam, KJ, Marghoob, AA, Scope, A, Dusza, SW, Cordova, MA, Fonseca, M, Wu, X & Halpern, AC 2014, 'Melanocytic naevi with globular and reticular dermoscopic patterns display distinct BRAF V600E expression profiles and histopathological patterns', British Journal of Dermatology, vol. 171, no. 5, pp. 1060-1065. https://doi.org/10.1111/bjd.13260
Marchetti, M. A. ; Kiuru, Maija Ht ; Busam, K. J. ; Marghoob, A. A. ; Scope, A. ; Dusza, S. W. ; Cordova, M. A. ; Fonseca, M. ; Wu, X. ; Halpern, A. C. / Melanocytic naevi with globular and reticular dermoscopic patterns display distinct BRAF V600E expression profiles and histopathological patterns. In: British Journal of Dermatology. 2014 ; Vol. 171, No. 5. pp. 1060-1065.
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title = "Melanocytic naevi with globular and reticular dermoscopic patterns display distinct BRAF V600E expression profiles and histopathological patterns",
abstract = "Results BRAF V600E expression was detected in 11 of 12 globular naevi vs. four of 13 reticular naevi (91·7{\%} vs. 30·1{\%}, P = 0·004). A predominantly dermal growth pattern (P < 0·001) and the presence of large junctional nests (P = 0·017) were each associated with a globular dermoscopic pattern. The presence of either a predominantly dermal growth pattern or large junctional nests was found in 13 of 15 naevi positive for BRAF V600E and in two of 10 naevi negative for BRAF V600E (86·7{\%} vs. 20{\%}, P = 0·002).Conclusions The frequency of BRAF V600E mutations differs in naevi distinguished by unique dermoscopic structures and microanatomical growth patterns. Globular naevi, which most often histologically correspond to a predominantly dermal growth pattern and/or the presence of large junctional nests, are significantly more likely to express BRAF V600E than reticular naevi. These preliminary results require validation, but may directly inform future studies of naevogenesis and melanoma genesis. What's already known about this topic? Activating BRAF V600E mutations have been detected in a majority of melanocytic naevi. What does this study add? Dermoscopically defined globular naevi, which are most often composed of large melanocytic nests and a predominantly dermal growth pattern, are at least three times more likely to express BRAF V600E than dermoscopically defined reticular naevi.Methods We retrospectively identified histologically proven melanocytic naevi with banal reticular or globular dermoscopic patterns and evaluated BRAF V600E expression using immunohistochemistry.Summary Background BRAF (v-raf murine sarcoma viral oncogene homologue B) V600E mutations have been detected with high frequency in melanocytic naevi. Few studies have stratified analyses by naevus dermoscopic pattern.Objectives To determine the frequency of BRAF V600E expression and histopathological pattern in acquired melanocytic naevi distinguished by a globular vs. reticular dermoscopic pattern.",
author = "Marchetti, {M. A.} and Kiuru, {Maija Ht} and Busam, {K. J.} and Marghoob, {A. A.} and A. Scope and Dusza, {S. W.} and Cordova, {M. A.} and M. Fonseca and X. Wu and Halpern, {A. C.}",
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T1 - Melanocytic naevi with globular and reticular dermoscopic patterns display distinct BRAF V600E expression profiles and histopathological patterns

AU - Marchetti, M. A.

AU - Kiuru, Maija Ht

AU - Busam, K. J.

AU - Marghoob, A. A.

AU - Scope, A.

AU - Dusza, S. W.

AU - Cordova, M. A.

AU - Fonseca, M.

AU - Wu, X.

AU - Halpern, A. C.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Results BRAF V600E expression was detected in 11 of 12 globular naevi vs. four of 13 reticular naevi (91·7% vs. 30·1%, P = 0·004). A predominantly dermal growth pattern (P < 0·001) and the presence of large junctional nests (P = 0·017) were each associated with a globular dermoscopic pattern. The presence of either a predominantly dermal growth pattern or large junctional nests was found in 13 of 15 naevi positive for BRAF V600E and in two of 10 naevi negative for BRAF V600E (86·7% vs. 20%, P = 0·002).Conclusions The frequency of BRAF V600E mutations differs in naevi distinguished by unique dermoscopic structures and microanatomical growth patterns. Globular naevi, which most often histologically correspond to a predominantly dermal growth pattern and/or the presence of large junctional nests, are significantly more likely to express BRAF V600E than reticular naevi. These preliminary results require validation, but may directly inform future studies of naevogenesis and melanoma genesis. What's already known about this topic? Activating BRAF V600E mutations have been detected in a majority of melanocytic naevi. What does this study add? Dermoscopically defined globular naevi, which are most often composed of large melanocytic nests and a predominantly dermal growth pattern, are at least three times more likely to express BRAF V600E than dermoscopically defined reticular naevi.Methods We retrospectively identified histologically proven melanocytic naevi with banal reticular or globular dermoscopic patterns and evaluated BRAF V600E expression using immunohistochemistry.Summary Background BRAF (v-raf murine sarcoma viral oncogene homologue B) V600E mutations have been detected with high frequency in melanocytic naevi. Few studies have stratified analyses by naevus dermoscopic pattern.Objectives To determine the frequency of BRAF V600E expression and histopathological pattern in acquired melanocytic naevi distinguished by a globular vs. reticular dermoscopic pattern.

AB - Results BRAF V600E expression was detected in 11 of 12 globular naevi vs. four of 13 reticular naevi (91·7% vs. 30·1%, P = 0·004). A predominantly dermal growth pattern (P < 0·001) and the presence of large junctional nests (P = 0·017) were each associated with a globular dermoscopic pattern. The presence of either a predominantly dermal growth pattern or large junctional nests was found in 13 of 15 naevi positive for BRAF V600E and in two of 10 naevi negative for BRAF V600E (86·7% vs. 20%, P = 0·002).Conclusions The frequency of BRAF V600E mutations differs in naevi distinguished by unique dermoscopic structures and microanatomical growth patterns. Globular naevi, which most often histologically correspond to a predominantly dermal growth pattern and/or the presence of large junctional nests, are significantly more likely to express BRAF V600E than reticular naevi. These preliminary results require validation, but may directly inform future studies of naevogenesis and melanoma genesis. What's already known about this topic? Activating BRAF V600E mutations have been detected in a majority of melanocytic naevi. What does this study add? Dermoscopically defined globular naevi, which are most often composed of large melanocytic nests and a predominantly dermal growth pattern, are at least three times more likely to express BRAF V600E than dermoscopically defined reticular naevi.Methods We retrospectively identified histologically proven melanocytic naevi with banal reticular or globular dermoscopic patterns and evaluated BRAF V600E expression using immunohistochemistry.Summary Background BRAF (v-raf murine sarcoma viral oncogene homologue B) V600E mutations have been detected with high frequency in melanocytic naevi. Few studies have stratified analyses by naevus dermoscopic pattern.Objectives To determine the frequency of BRAF V600E expression and histopathological pattern in acquired melanocytic naevi distinguished by a globular vs. reticular dermoscopic pattern.

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