Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae

Jacqueline Schmuckli-Maurer, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Rad54 protein is an important component of the recombinational DNA repair pathway in vegetative Saccharomyces cerevisiae cells. Unlike those in other members of the RAD52 group, the meiotic defect in rad54 is rather mild, reducing spore viability only to 26-65%. A consistently greater requirement for Rad54p during meiosis was observed in hybrid strains, suggesting that Rad54p has a certain role in interhomolog interactions. Such a role is probably minor as no recombination defects were found in the surviving gametes in three genetic intervals on chromosome V. Also, the spore viability pattern in tetrads did not reflect an increase in nondisjunction at meiosis I indicative of a meiotic recombination defect. We suggest that the meiotic defect of rad54 cells lies in the failure to repair meiosis-specific double-strand breaks outside the context of the highly differentiated pathway leading to interhomolog joint molecules and meiotic crossovers that ensure accurate segregation at meiosis I.

Original languageEnglish (US)
Pages (from-to)86-93
Number of pages8
JournalChromosoma
Volume109
Issue number1-2
DOIs
StatePublished - 2000

Fingerprint

Meiosis
Genetic Recombination
Saccharomyces cerevisiae
Spores
Recombinational DNA Repair
Germ Cells
Chromosomes
Joints
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae. / Schmuckli-Maurer, Jacqueline; Heyer, Wolf Dietrich.

In: Chromosoma, Vol. 109, No. 1-2, 2000, p. 86-93.

Research output: Contribution to journalArticle

Schmuckli-Maurer, Jacqueline ; Heyer, Wolf Dietrich. / Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae. In: Chromosoma. 2000 ; Vol. 109, No. 1-2. pp. 86-93.
@article{bdfe5be451fb44f19f4982043d5e50de,
title = "Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae",
abstract = "The Rad54 protein is an important component of the recombinational DNA repair pathway in vegetative Saccharomyces cerevisiae cells. Unlike those in other members of the RAD52 group, the meiotic defect in rad54 is rather mild, reducing spore viability only to 26-65{\%}. A consistently greater requirement for Rad54p during meiosis was observed in hybrid strains, suggesting that Rad54p has a certain role in interhomolog interactions. Such a role is probably minor as no recombination defects were found in the surviving gametes in three genetic intervals on chromosome V. Also, the spore viability pattern in tetrads did not reflect an increase in nondisjunction at meiosis I indicative of a meiotic recombination defect. We suggest that the meiotic defect of rad54 cells lies in the failure to repair meiosis-specific double-strand breaks outside the context of the highly differentiated pathway leading to interhomolog joint molecules and meiotic crossovers that ensure accurate segregation at meiosis I.",
author = "Jacqueline Schmuckli-Maurer and Heyer, {Wolf Dietrich}",
year = "2000",
doi = "10.1007/s004120050415",
language = "English (US)",
volume = "109",
pages = "86--93",
journal = "Chromosoma",
issn = "0009-5915",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "1-2",

}

TY - JOUR

T1 - Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae

AU - Schmuckli-Maurer, Jacqueline

AU - Heyer, Wolf Dietrich

PY - 2000

Y1 - 2000

N2 - The Rad54 protein is an important component of the recombinational DNA repair pathway in vegetative Saccharomyces cerevisiae cells. Unlike those in other members of the RAD52 group, the meiotic defect in rad54 is rather mild, reducing spore viability only to 26-65%. A consistently greater requirement for Rad54p during meiosis was observed in hybrid strains, suggesting that Rad54p has a certain role in interhomolog interactions. Such a role is probably minor as no recombination defects were found in the surviving gametes in three genetic intervals on chromosome V. Also, the spore viability pattern in tetrads did not reflect an increase in nondisjunction at meiosis I indicative of a meiotic recombination defect. We suggest that the meiotic defect of rad54 cells lies in the failure to repair meiosis-specific double-strand breaks outside the context of the highly differentiated pathway leading to interhomolog joint molecules and meiotic crossovers that ensure accurate segregation at meiosis I.

AB - The Rad54 protein is an important component of the recombinational DNA repair pathway in vegetative Saccharomyces cerevisiae cells. Unlike those in other members of the RAD52 group, the meiotic defect in rad54 is rather mild, reducing spore viability only to 26-65%. A consistently greater requirement for Rad54p during meiosis was observed in hybrid strains, suggesting that Rad54p has a certain role in interhomolog interactions. Such a role is probably minor as no recombination defects were found in the surviving gametes in three genetic intervals on chromosome V. Also, the spore viability pattern in tetrads did not reflect an increase in nondisjunction at meiosis I indicative of a meiotic recombination defect. We suggest that the meiotic defect of rad54 cells lies in the failure to repair meiosis-specific double-strand breaks outside the context of the highly differentiated pathway leading to interhomolog joint molecules and meiotic crossovers that ensure accurate segregation at meiosis I.

UR - http://www.scopus.com/inward/record.url?scp=0033933947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033933947&partnerID=8YFLogxK

U2 - 10.1007/s004120050415

DO - 10.1007/s004120050415

M3 - Article

C2 - 10855498

AN - SCOPUS:0033933947

VL - 109

SP - 86

EP - 93

JO - Chromosoma

JF - Chromosoma

SN - 0009-5915

IS - 1-2

ER -