Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary

Ripla Arora, Emilie Abby, Adam D J Ross, Andrea V. Cantu, Michael D. Kissner, Vianca Castro, Hsin-Yi Henry Ho, Gabriel Livera, Diana J. Laird

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells to initiation or propagation of meiosis. In a Ror2 mutant with diminished germ cell number and migration, we find that overall timing of meiotic initiation is delayed at the population level. We use chemotherapeutic depletion to exclude a profoundly reduced number of germ cells as a cause for meiotic delay.We rule out sex reversal or failure to specify somatic support cells as contributors to the meiotic phenotype. Instead, we find that anomalies in the distribution of germ cells as well as gonad shape in mutants contribute to aberrant initiation of meiosis. Our analysis supports a model of meiotic initiation via diffusible signal(s), excludes a role for germ cells in commencing the meiotic wave and furnishes the first phenotypic demonstration of the wave of meiotic entry. Finally, our studies underscore the importance of considering germ cell migration defects while studying meiosis to discern secondary effects resulting from positioning versus primary meiotic entry phenotypes.

Original languageEnglish (US)
Pages (from-to)2493-2499
Number of pages7
JournalJournal of Cell Science
Volume129
Issue number13
DOIs
StatePublished - 2016

Fingerprint

Germ Cells
Ovary
Cell Count
Meiosis
Cell Movement
Phenotype
Gonads
Population

Keywords

  • Germ cell
  • Gonad
  • Meiosis
  • Migration
  • Ror2
  • Wave

ASJC Scopus subject areas

  • Cell Biology

Cite this

Arora, R., Abby, E., Ross, A. D. J., Cantu, A. V., Kissner, M. D., Castro, V., ... Laird, D. J. (2016). Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary. Journal of Cell Science, 129(13), 2493-2499. https://doi.org/10.1242/jcs.189910

Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary. / Arora, Ripla; Abby, Emilie; Ross, Adam D J; Cantu, Andrea V.; Kissner, Michael D.; Castro, Vianca; Ho, Hsin-Yi Henry; Livera, Gabriel; Laird, Diana J.

In: Journal of Cell Science, Vol. 129, No. 13, 2016, p. 2493-2499.

Research output: Contribution to journalArticle

Arora, R, Abby, E, Ross, ADJ, Cantu, AV, Kissner, MD, Castro, V, Ho, H-YH, Livera, G & Laird, DJ 2016, 'Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary', Journal of Cell Science, vol. 129, no. 13, pp. 2493-2499. https://doi.org/10.1242/jcs.189910
Arora, Ripla ; Abby, Emilie ; Ross, Adam D J ; Cantu, Andrea V. ; Kissner, Michael D. ; Castro, Vianca ; Ho, Hsin-Yi Henry ; Livera, Gabriel ; Laird, Diana J. / Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary. In: Journal of Cell Science. 2016 ; Vol. 129, No. 13. pp. 2493-2499.
@article{cfebce46a4f6463b943b290eb268ccae,
title = "Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary",
abstract = "Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells to initiation or propagation of meiosis. In a Ror2 mutant with diminished germ cell number and migration, we find that overall timing of meiotic initiation is delayed at the population level. We use chemotherapeutic depletion to exclude a profoundly reduced number of germ cells as a cause for meiotic delay.We rule out sex reversal or failure to specify somatic support cells as contributors to the meiotic phenotype. Instead, we find that anomalies in the distribution of germ cells as well as gonad shape in mutants contribute to aberrant initiation of meiosis. Our analysis supports a model of meiotic initiation via diffusible signal(s), excludes a role for germ cells in commencing the meiotic wave and furnishes the first phenotypic demonstration of the wave of meiotic entry. Finally, our studies underscore the importance of considering germ cell migration defects while studying meiosis to discern secondary effects resulting from positioning versus primary meiotic entry phenotypes.",
keywords = "Germ cell, Gonad, Meiosis, Migration, Ror2, Wave",
author = "Ripla Arora and Emilie Abby and Ross, {Adam D J} and Cantu, {Andrea V.} and Kissner, {Michael D.} and Vianca Castro and Ho, {Hsin-Yi Henry} and Gabriel Livera and Laird, {Diana J.}",
year = "2016",
doi = "10.1242/jcs.189910",
language = "English (US)",
volume = "129",
pages = "2493--2499",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "13",

}

TY - JOUR

T1 - Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary

AU - Arora, Ripla

AU - Abby, Emilie

AU - Ross, Adam D J

AU - Cantu, Andrea V.

AU - Kissner, Michael D.

AU - Castro, Vianca

AU - Ho, Hsin-Yi Henry

AU - Livera, Gabriel

AU - Laird, Diana J.

PY - 2016

Y1 - 2016

N2 - Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells to initiation or propagation of meiosis. In a Ror2 mutant with diminished germ cell number and migration, we find that overall timing of meiotic initiation is delayed at the population level. We use chemotherapeutic depletion to exclude a profoundly reduced number of germ cells as a cause for meiotic delay.We rule out sex reversal or failure to specify somatic support cells as contributors to the meiotic phenotype. Instead, we find that anomalies in the distribution of germ cells as well as gonad shape in mutants contribute to aberrant initiation of meiosis. Our analysis supports a model of meiotic initiation via diffusible signal(s), excludes a role for germ cells in commencing the meiotic wave and furnishes the first phenotypic demonstration of the wave of meiotic entry. Finally, our studies underscore the importance of considering germ cell migration defects while studying meiosis to discern secondary effects resulting from positioning versus primary meiotic entry phenotypes.

AB - Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells to initiation or propagation of meiosis. In a Ror2 mutant with diminished germ cell number and migration, we find that overall timing of meiotic initiation is delayed at the population level. We use chemotherapeutic depletion to exclude a profoundly reduced number of germ cells as a cause for meiotic delay.We rule out sex reversal or failure to specify somatic support cells as contributors to the meiotic phenotype. Instead, we find that anomalies in the distribution of germ cells as well as gonad shape in mutants contribute to aberrant initiation of meiosis. Our analysis supports a model of meiotic initiation via diffusible signal(s), excludes a role for germ cells in commencing the meiotic wave and furnishes the first phenotypic demonstration of the wave of meiotic entry. Finally, our studies underscore the importance of considering germ cell migration defects while studying meiosis to discern secondary effects resulting from positioning versus primary meiotic entry phenotypes.

KW - Germ cell

KW - Gonad

KW - Meiosis

KW - Migration

KW - Ror2

KW - Wave

UR - http://www.scopus.com/inward/record.url?scp=84977652556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977652556&partnerID=8YFLogxK

U2 - 10.1242/jcs.189910

DO - 10.1242/jcs.189910

M3 - Article

C2 - 27199373

AN - SCOPUS:84977652556

VL - 129

SP - 2493

EP - 2499

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 13

ER -