Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport

T. L Haut Donahue, T. R. Haut, Clare E Yellowley-genetos, H. J. Donahue, C. R. Jacobs

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Fluid flow has been shown to be a potent physical stimulus in the regulation of bone cell metabolism. In addition to membrane shear stress, loading-induced fluid flow will enhance chemotransport due to convection or mass transport thereby affecting the biochemical environment surrounding the cell. This study investigated the role of oscillating fluid flow induced shear stress and chemotransport in cellular mechanotransduction mechanisms in bone. Intracellular calcium mobilization and prostaglandin E2 (PGE2) production were studied with varying levels of shear stress and chemotransport. In this study MC3T3-E1 cells responded to oscillating fluid flow with both an increase in intracellular calcium concentration ([Ca2+]i) and an increase in PGE2 production. These fluid flow induced responses were modulated by chemotransport. The percentage of cells responding with an [Ca2+]i oscillation increased with increasing flow rate, as did the production of PGE2. In addition, depriving the cells of nutrients during fluid flow resulted in an inhibition of both [Ca2+]i mobilization and PGE2 production. These data suggest that depriving the cells of a yet to be determined biochemical factor in media affects the responsiveness of bone cells even at a constant peak shear stress. Chemotransport alone will not elicit a response, but it appears that sufficient nutrient supply or waste removal is needed for the response to oscillating fluid flow induced shear stress.

Original languageEnglish (US)
Pages (from-to)1363-1371
Number of pages9
JournalJournal of Biomechanics
Volume36
Issue number9
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Shear stress
Flow of fluids
Bone
Bone and Bones
Dinoprostone
Nutrients
Calcium
Cellular Mechanotransduction
Food
Convection
Metabolism
Mass transfer
Flow rate
Membranes

Keywords

  • Bone
  • Calcium
  • Fluid flow
  • Mechanotransduction
  • Shear stress

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport. / Donahue, T. L Haut; Haut, T. R.; Yellowley-genetos, Clare E; Donahue, H. J.; Jacobs, C. R.

In: Journal of Biomechanics, Vol. 36, No. 9, 01.09.2003, p. 1363-1371.

Research output: Contribution to journalArticle

Donahue, T. L Haut ; Haut, T. R. ; Yellowley-genetos, Clare E ; Donahue, H. J. ; Jacobs, C. R. / Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport. In: Journal of Biomechanics. 2003 ; Vol. 36, No. 9. pp. 1363-1371.
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