Mechanistic studies of the degradation of juvenile hormone esterase in Manduca sexta

Bryony C. Bonning, Tim F. Booth, Brace D. Hammock

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mechanisms of degradation of juvenile hormone esterase (JHE) were investigated in larvae of the tobacco hornworm, Manduca sexta. JHE is removed from the hemolymph by the pericardial cells by receptor-mediated endocytosis and is ultimately degraded in the lysosomes. Immunoprecipitation experiments and native PAGE followed by Western blotting showed that JHE associates with a putative heat shock cognate protein (Hsp). Approximately 25% of the active JHE in the pericardial cell complex is associated with the putative Hsp 1 h postinjection of affinity purified JHE. Electron microscope analysis revealed that the putative Hsp is located in the trans-Golgi network of pericardial cells, where it is hypothesized to be involved in sorting of proteins destined for the lysosomes, from those destined for the cell membrane. Data acquired from immunoprecipitation and Western blotting experiments argue against the involvement of ubiquitin in the degradation of JHE. Injection of radiolabeled JHE into larvae of M. sexta followed by SDS-PAGE of pericardial cell homogenates revealed covalent binding of an unidentified protein to JHE in the pericardial cell complex.

Original languageEnglish (US)
Pages (from-to)275-286
Number of pages12
JournalArchives of Insect Biochemistry and Physiology
Volume34
Issue number3
StatePublished - 1997

Fingerprint

juvenile hormone esterase
Manduca
Manduca sexta
Degradation
degradation
Heat-Shock Proteins
heat shock proteins
lysosomes
Lysosomes
Immunoprecipitation
Larva
cells
Western blotting
Proteins
Western Blotting
trans-Golgi Network
Native Polyacrylamide Gel Electrophoresis
Tobacco
protein transport
Hemolymph

Keywords

  • Heat shock protein
  • Juvenile hormone esterase
  • Manduca sexta
  • Protein degradation

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

Cite this

Mechanistic studies of the degradation of juvenile hormone esterase in Manduca sexta. / Bonning, Bryony C.; Booth, Tim F.; Hammock, Brace D.

In: Archives of Insect Biochemistry and Physiology, Vol. 34, No. 3, 1997, p. 275-286.

Research output: Contribution to journalArticle

Bonning, Bryony C. ; Booth, Tim F. ; Hammock, Brace D. / Mechanistic studies of the degradation of juvenile hormone esterase in Manduca sexta. In: Archives of Insect Biochemistry and Physiology. 1997 ; Vol. 34, No. 3. pp. 275-286.
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