Abstract
Nanoporous gold (np-Au) is a promising multifunctional material for neural electrodes. We have previously shown that np-Au nanotopography reduces astrocyte surface coverage (linked to undesirable gliosis) while maintaining high neuronal coverage in a cortical primary neuron-glia co-culture model as long as 2 weeks in vitro. Here, we investigate the potential influence of secreted soluble factors from cells grown on np-Au leading to the cell type-specific surface coverage on conventional tissue culture plastic. We then test the hypothesis that secretion of factors is responsible for inhibiting astrocyte coverage on np-Au. In order to assess whether factors secreted from cells grown on np-Au surfaces reduced surface coverage by astrocytes, we seeded fresh primary rat neuron-glia co-cultures on conventional polystyrene culture dishes, but maintained the cells in conditioned media from co-cultures grown on np-Au surfaces. After 1 week in vitro, a preferential reduction in astrocyte surface coverage was not observed, suggesting that soluble factors are not playing a role. In contrast, 4 h after cell seeding there were a significant number of non-adhered, yet still viable, cells for the cultures on np-Au surfaces. We hypothesize that the non-adherent cells are mainly astrocytes, because: (i) there was no difference in neuronal cell coverage between np-Au and pl-Au for long culture durations and (ii) neurons are post-mitotic and not expected to increase in number upon attaching to the surface. Overall, the results suggest that the np-Au topography leads to preferential neuronal attachment shortly after cell seeding and limits astrocyte-specific np-Au surface coverage at longer culture durations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 433-442 |
| Number of pages | 10 |
| Journal | Cellular and Molecular Bioengineering |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2016 |
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Keywords
- Cell attachment
- Cell-material interaction
- Gliosis
- Nanoporous gold
- Nanostructure
- Neuroengineering
- Neuron-glia co-culture
- Soluble factors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Modeling and Simulation
Cite this
Mechanisms of Reduced Astrocyte Surface Coverage in Cortical Neuron-Glia Co-cultures on Nanoporous Gold Surfaces. / Chapman, Christopher A R; Chen, Hao; Stamou, Marianna; Lein, Pamela J; Seker, Erkin.
In: Cellular and Molecular Bioengineering, Vol. 9, No. 3, 01.09.2016, p. 433-442.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanisms of Reduced Astrocyte Surface Coverage in Cortical Neuron-Glia Co-cultures on Nanoporous Gold Surfaces
AU - Chapman, Christopher A R
AU - Chen, Hao
AU - Stamou, Marianna
AU - Lein, Pamela J
AU - Seker, Erkin
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Nanoporous gold (np-Au) is a promising multifunctional material for neural electrodes. We have previously shown that np-Au nanotopography reduces astrocyte surface coverage (linked to undesirable gliosis) while maintaining high neuronal coverage in a cortical primary neuron-glia co-culture model as long as 2 weeks in vitro. Here, we investigate the potential influence of secreted soluble factors from cells grown on np-Au leading to the cell type-specific surface coverage on conventional tissue culture plastic. We then test the hypothesis that secretion of factors is responsible for inhibiting astrocyte coverage on np-Au. In order to assess whether factors secreted from cells grown on np-Au surfaces reduced surface coverage by astrocytes, we seeded fresh primary rat neuron-glia co-cultures on conventional polystyrene culture dishes, but maintained the cells in conditioned media from co-cultures grown on np-Au surfaces. After 1 week in vitro, a preferential reduction in astrocyte surface coverage was not observed, suggesting that soluble factors are not playing a role. In contrast, 4 h after cell seeding there were a significant number of non-adhered, yet still viable, cells for the cultures on np-Au surfaces. We hypothesize that the non-adherent cells are mainly astrocytes, because: (i) there was no difference in neuronal cell coverage between np-Au and pl-Au for long culture durations and (ii) neurons are post-mitotic and not expected to increase in number upon attaching to the surface. Overall, the results suggest that the np-Au topography leads to preferential neuronal attachment shortly after cell seeding and limits astrocyte-specific np-Au surface coverage at longer culture durations.
AB - Nanoporous gold (np-Au) is a promising multifunctional material for neural electrodes. We have previously shown that np-Au nanotopography reduces astrocyte surface coverage (linked to undesirable gliosis) while maintaining high neuronal coverage in a cortical primary neuron-glia co-culture model as long as 2 weeks in vitro. Here, we investigate the potential influence of secreted soluble factors from cells grown on np-Au leading to the cell type-specific surface coverage on conventional tissue culture plastic. We then test the hypothesis that secretion of factors is responsible for inhibiting astrocyte coverage on np-Au. In order to assess whether factors secreted from cells grown on np-Au surfaces reduced surface coverage by astrocytes, we seeded fresh primary rat neuron-glia co-cultures on conventional polystyrene culture dishes, but maintained the cells in conditioned media from co-cultures grown on np-Au surfaces. After 1 week in vitro, a preferential reduction in astrocyte surface coverage was not observed, suggesting that soluble factors are not playing a role. In contrast, 4 h after cell seeding there were a significant number of non-adhered, yet still viable, cells for the cultures on np-Au surfaces. We hypothesize that the non-adherent cells are mainly astrocytes, because: (i) there was no difference in neuronal cell coverage between np-Au and pl-Au for long culture durations and (ii) neurons are post-mitotic and not expected to increase in number upon attaching to the surface. Overall, the results suggest that the np-Au topography leads to preferential neuronal attachment shortly after cell seeding and limits astrocyte-specific np-Au surface coverage at longer culture durations.
KW - Cell attachment
KW - Cell-material interaction
KW - Gliosis
KW - Nanoporous gold
KW - Nanostructure
KW - Neuroengineering
KW - Neuron-glia co-culture
KW - Soluble factors
UR - http://www.scopus.com/inward/record.url?scp=84973126993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973126993&partnerID=8YFLogxK
U2 - 10.1007/s12195-016-0449-4
DO - 10.1007/s12195-016-0449-4
M3 - Article
AN - SCOPUS:84973126993
VL - 9
SP - 433
EP - 442
JO - Cellular and Molecular Bioengineering
JF - Cellular and Molecular Bioengineering
SN - 1865-5025
IS - 3
ER -