Mechanisms for de novo biogenesis of an apical membrane compartment in groups of simple epithelial cells surrounded by extracellular matrix

George K. Ojakian, W. James Nelson, Kenneth A Beck

Research output: Contribution to journalArticle

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Abstract

In open monolayers of epithelial cells grown in vitro, the apical membrane domain forms on the free cell surface that faces the culture medium. However, in vivo, the apical lumenal compartment arises within groups of cells that do not have a free cell surface. We designed in vitro culture conditions, using small colonies of MDCK cells overlaid with collagen, in which formation of the apical membrane must occur de novo by remodeling existing membrane domains that are contacted by other cells or extracellular matrix. Within 12 hours of collagen overlay, the apical membrane glycoprotein gp135 is removed from the free cell surface, while lateral membrane proteins (e.g. Na+,K+-ATPase) remain at sites of cell-cell contacts. Subsequently, lumenal structures, containing gp135 and the apically secreted protein gp81, formed within these cell-cell contacts. Na+,K+-ATPase, adherens junction (E-cadherin, α- and β-catenins) and tight junction (ZO-1) proteins were localized on the lateral membrane adjacent to, but excluded from the gp135-positive lumenal compartment. Therefore, each lumen represents a newly formed apical compartment on the lateral membrane. The Golgi complex (α-mannosidase II and Golgi β-spectrin), centrosomes (γ-tubulin) and microtubules reorient to a cytoplasmic position adjacent to the newly-forming apical lumenal compartments. Significantly, addition of colchicine, nocodazole or brefeldin A inhibits apical lumen formation. These results demonstrate that simple epithelial cells form an apical lumenal compartment de novo through initial intermixing, and then sorting of apical and basal-lateral membrane proteins at sites of cell-cell contacts. In addition, apical lumen formation requires an intact microtubule network, microtubule-dependent reorientation of the Golgi complex and secretory apparatus, and fully functional protein delivery from the Golgi complex to the forming apical cell surface.

Original languageEnglish (US)
Pages (from-to)2781-2794
Number of pages14
JournalJournal of Cell Science
Volume110
Issue number22
StatePublished - 1997
Externally publishedYes

Fingerprint

Extracellular Matrix
Epithelial Cells
Membranes
Golgi Apparatus
Microtubules
Contactin 1
Membrane Proteins
Collagen
Zonula Occludens-1 Protein
Nocodazole
Brefeldin A
Adherens Junctions
Catenins
Spectrin
Centrosome
Madin Darby Canine Kidney Cells
Membrane Glycoproteins
Colchicine
Cadherins
Tubulin

Keywords

  • Centrosome
  • Epithelial polarity
  • Golgi
  • Microtubule

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mechanisms for de novo biogenesis of an apical membrane compartment in groups of simple epithelial cells surrounded by extracellular matrix. / Ojakian, George K.; Nelson, W. James; Beck, Kenneth A.

In: Journal of Cell Science, Vol. 110, No. 22, 1997, p. 2781-2794.

Research output: Contribution to journalArticle

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