Mechanism of the induction of brain c-Fos-positive neurons by lipid absorption

Chun Min Lo, Liyun Ma, Ming Zhang Dian, Rachel Lee, Abby Qin, Min Liu, Stephen C. Woods, Randall R. Sakai, Helen E Raybould, Patrick Tso

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Many gastrointestinal meal-related signals are transmitted to the central nervous system via the vagus nerve and thereby control changes in meal size. The c-Fos-positive neuron has been used as a marker of neuronal activation after lipid meals to examine the contribution of a selective macronutrient on brain neurocircuit activity. In rats fed Intralipid, the c-Fos-positive neurons were highly stimulated in the nucleus of the solitary tract (NTS) and in the hypothalamus, including the paraventricular nucleus (PVN), arcuate nucleus of the hypothalamus (ARC), and ventromedial hypothalamus at 4 h lipid feeding. However, c-Fos-like immunoreactivity was markedly attenuated in these brain regions when chylomicron formation/secretion was blocked by Pluronic L-81. After lymph was diverted from the lymph cannulated animals, the rats had a lower number of c-Fos-positive cells in the NTS and ARC. In contrast, the rats had higher c-Fos-positive neurons in PVN. The present study also revealed that c-Fos-positive neurons induced by feeding of Intalipid were abolished by CCK type 1 receptor antagonist, Lorglumide. We conclude that the formation and/or secretion of chylomicron are critical steps for initiating neuronal activation in the brain.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume292
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Cholecystokinin type 1 receptor antagonist
  • Chylomicron
  • Hindbrain
  • Hypothalamus

ASJC Scopus subject areas

  • Physiology

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