Mechanism of shape determination in motile cells

Kinneret Keren, Zachary Pincus, Greg M. Allen, Erin L. Barnhart, Gerard Marriott, Alex Mogilner, Julie A. Theriot

Research output: Contribution to journalArticle

388 Citations (Scopus)

Abstract

The shape of motile cells is determined by many dynamic processes spanning several orders of magnitude in space and time, from local polymerization of actin monomers at subsecond timescales to global, cell-scale geometry that may persist for hours. Understanding the mechanism of shape determination in cells has proved to be extremely challenging due to the numerous components involved and the complexity of their interactions. Here we harness the natural phenotypic variability in a large population of motile epithelial keratocytes from fish (Hypsophrys nicaraguensis) to reveal mechanisms of shape determination. We find that the cells inhabit a low-dimensional, highly correlated spectrum of possible functional states. We further show that a model of actin network treadmilling in an inextensible membrane bag can quantitatively recapitulate this spectrum and predict both cell shape and speed. Our model provides a simple biochemical and biophysical basis for the observed morphology and behaviour of motile cells.

Original languageEnglish (US)
Pages (from-to)475-480
Number of pages6
JournalNature
Volume453
Issue number7194
DOIs
StatePublished - May 22 2008

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Cell Shape
Actins
Polymerization
Fishes
Membranes
Population

ASJC Scopus subject areas

  • General

Cite this

Keren, K., Pincus, Z., Allen, G. M., Barnhart, E. L., Marriott, G., Mogilner, A., & Theriot, J. A. (2008). Mechanism of shape determination in motile cells. Nature, 453(7194), 475-480. https://doi.org/10.1038/nature06952

Mechanism of shape determination in motile cells. / Keren, Kinneret; Pincus, Zachary; Allen, Greg M.; Barnhart, Erin L.; Marriott, Gerard; Mogilner, Alex; Theriot, Julie A.

In: Nature, Vol. 453, No. 7194, 22.05.2008, p. 475-480.

Research output: Contribution to journalArticle

Keren, K, Pincus, Z, Allen, GM, Barnhart, EL, Marriott, G, Mogilner, A & Theriot, JA 2008, 'Mechanism of shape determination in motile cells', Nature, vol. 453, no. 7194, pp. 475-480. https://doi.org/10.1038/nature06952
Keren K, Pincus Z, Allen GM, Barnhart EL, Marriott G, Mogilner A et al. Mechanism of shape determination in motile cells. Nature. 2008 May 22;453(7194):475-480. https://doi.org/10.1038/nature06952
Keren, Kinneret ; Pincus, Zachary ; Allen, Greg M. ; Barnhart, Erin L. ; Marriott, Gerard ; Mogilner, Alex ; Theriot, Julie A. / Mechanism of shape determination in motile cells. In: Nature. 2008 ; Vol. 453, No. 7194. pp. 475-480.
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