Abstract
Background - Human embryonic stem cells (hESCs) can be efficiently and reproducibly directed into cardiomyocytes (CMs) using stage-specific induction protocols. However, their functional properties and suitability for clinical and other applications have not been evaluated. Methods and Results - Here we showed that CMs derived from multiple pluripotent human stem cell lines (hESC: H1, HES2) and types (induced pluripotent stem cell) using different in vitro differentiation protocols (embryoid body formation, endodermal induction, directed differentiation) commonly displayed immature, proarrhythmic action potential properties such as high degree of automaticity, depolarized resting membrane potential, Phase 4-depolarization, and delayed after-depolarization. Among the panoply of sarcolemmal ionic currents investigated (INa +/ICaL +/IKr +/I NCX +/If +/Ito +/IK1 -/IKs -), we pinpointed the lack of the Kir2.1-encoded inwardly rectifying K+ current (I K1) as the single mechanistic contributor to the observed immature electrophysiological properties in hESC-CMs. Forced expression of Kir2.1 in hESC-CMs led to robust expression of Ba2+-sensitive IK1 and, more importantly, completely ablated all the proarrhythmic action potential traits, rendering the electrophysiological phenotype indistinguishable from the adult counterparts. These results provided the first link of a complex developmentally arrested phenotype to a major effector gene, and importantly, further led us to develop a bio-mimetic culturing strategy for enhancing maturation. Conclusions - By providing the environmental cues that are missing in conventional culturing method, this approach did not require any genetic or pharmacological interventions. Our findings can facilitate clinical applications, drug discovery, and cardiotoxicity screening by improving the yield, safety, and efficacy of derived CMs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 191-201 |
| Number of pages | 11 |
| Journal | Circulation: Arrhythmia and Electrophysiology |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2013 |
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Keywords
- Cardiac
- Electrophysiology
- Human
- Myocytes
- Pluripotent stem cells
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
- Medicine(all)
Cite this
Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes. / Lieu, Deborah; Fu, Ji Dong; Chiamvimonvat, Nipavan; Tung, Kelvin Chan; McNerney, Gregory P.; Huser, Thomas R; Keller, Gordon; Kong, Chi Wing; Li, Ronald A.
In: Circulation: Arrhythmia and Electrophysiology, Vol. 6, No. 1, 02.2013, p. 191-201.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes
AU - Lieu, Deborah
AU - Fu, Ji Dong
AU - Chiamvimonvat, Nipavan
AU - Tung, Kelvin Chan
AU - McNerney, Gregory P.
AU - Huser, Thomas R
AU - Keller, Gordon
AU - Kong, Chi Wing
AU - Li, Ronald A.
PY - 2013/2
Y1 - 2013/2
N2 - Background - Human embryonic stem cells (hESCs) can be efficiently and reproducibly directed into cardiomyocytes (CMs) using stage-specific induction protocols. However, their functional properties and suitability for clinical and other applications have not been evaluated. Methods and Results - Here we showed that CMs derived from multiple pluripotent human stem cell lines (hESC: H1, HES2) and types (induced pluripotent stem cell) using different in vitro differentiation protocols (embryoid body formation, endodermal induction, directed differentiation) commonly displayed immature, proarrhythmic action potential properties such as high degree of automaticity, depolarized resting membrane potential, Phase 4-depolarization, and delayed after-depolarization. Among the panoply of sarcolemmal ionic currents investigated (INa +/ICaL +/IKr +/I NCX +/If +/Ito +/IK1 -/IKs -), we pinpointed the lack of the Kir2.1-encoded inwardly rectifying K+ current (I K1) as the single mechanistic contributor to the observed immature electrophysiological properties in hESC-CMs. Forced expression of Kir2.1 in hESC-CMs led to robust expression of Ba2+-sensitive IK1 and, more importantly, completely ablated all the proarrhythmic action potential traits, rendering the electrophysiological phenotype indistinguishable from the adult counterparts. These results provided the first link of a complex developmentally arrested phenotype to a major effector gene, and importantly, further led us to develop a bio-mimetic culturing strategy for enhancing maturation. Conclusions - By providing the environmental cues that are missing in conventional culturing method, this approach did not require any genetic or pharmacological interventions. Our findings can facilitate clinical applications, drug discovery, and cardiotoxicity screening by improving the yield, safety, and efficacy of derived CMs.
AB - Background - Human embryonic stem cells (hESCs) can be efficiently and reproducibly directed into cardiomyocytes (CMs) using stage-specific induction protocols. However, their functional properties and suitability for clinical and other applications have not been evaluated. Methods and Results - Here we showed that CMs derived from multiple pluripotent human stem cell lines (hESC: H1, HES2) and types (induced pluripotent stem cell) using different in vitro differentiation protocols (embryoid body formation, endodermal induction, directed differentiation) commonly displayed immature, proarrhythmic action potential properties such as high degree of automaticity, depolarized resting membrane potential, Phase 4-depolarization, and delayed after-depolarization. Among the panoply of sarcolemmal ionic currents investigated (INa +/ICaL +/IKr +/I NCX +/If +/Ito +/IK1 -/IKs -), we pinpointed the lack of the Kir2.1-encoded inwardly rectifying K+ current (I K1) as the single mechanistic contributor to the observed immature electrophysiological properties in hESC-CMs. Forced expression of Kir2.1 in hESC-CMs led to robust expression of Ba2+-sensitive IK1 and, more importantly, completely ablated all the proarrhythmic action potential traits, rendering the electrophysiological phenotype indistinguishable from the adult counterparts. These results provided the first link of a complex developmentally arrested phenotype to a major effector gene, and importantly, further led us to develop a bio-mimetic culturing strategy for enhancing maturation. Conclusions - By providing the environmental cues that are missing in conventional culturing method, this approach did not require any genetic or pharmacological interventions. Our findings can facilitate clinical applications, drug discovery, and cardiotoxicity screening by improving the yield, safety, and efficacy of derived CMs.
KW - Cardiac
KW - Electrophysiology
KW - Human
KW - Myocytes
KW - Pluripotent stem cells
UR - http://www.scopus.com/inward/record.url?scp=84876322089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876322089&partnerID=8YFLogxK
U2 - 10.1161/CIRCEP.111.973420
DO - 10.1161/CIRCEP.111.973420
M3 - Article
C2 - 23392582
AN - SCOPUS:84876322089
VL - 6
SP - 191
EP - 201
JO - Circulation: Arrhythmia and Electrophysiology
JF - Circulation: Arrhythmia and Electrophysiology
SN - 1941-3149
IS - 1
ER -