Mechanics of neurophil phagocytosis: Experiments and quantitative models

Marc Herant, Volkmar Heinrich, Micah Dembo

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

To quantitatively characterize the mechanical processes that drive phagocytosis, we observed the FcγR-driven engulfment of antibody-coated beads of diameters 3 μm to 11 μm by initially spherical neutrophils. In particular, the time course of cell morphology, of bead motion and of cortical tension were determined. Here, we introduce a number of mechanistic models for phagocytosis and test their validity by comparing the experimental data with finite element computations for multiple bead sizes. We find that the optimal models involve two key mechanical interactions: a repulsion or pressure between cytoskeleton and free membrane that drives protrusion, and an attraction between cytoskeleton and membrane newly adherent to the bead that flattens the cell into a thin lamella. Other models such as cytoskeletal expansion or swelling appear to be ruled out as main drivers of phagocytosis because of the characteristics of bead motion during engulfment. We finally show that the protrusive force necessary for the engulfment of large beads points towards storage of strain energy in the cytoskeleton over a large distance from the leading edge (∼0.5 μm), and that the flattening force can plausibly be generated by the known concentrations of unconventional myosins at the leading edge.

Original languageEnglish (US)
Pages (from-to)1903-1913
Number of pages11
JournalJournal of Cell Science
Volume119
Issue number9
DOIs
StatePublished - May 1 2006

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Keywords

  • Cell mechanics
  • Cell motility
  • Finite element simulations
  • Lamella
  • Membrane tension

ASJC Scopus subject areas

  • Cell Biology

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