Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow

Scott I. Simon, Yu Hu, C. Wayne Smith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The functional significance of E-selectin binding to neutrophils under shear flow in the absence of chemotactic stimulation is examined. Two experimental settings were used. One simulates the potential influence of hydrodynamic shear on intercellular adhesive interactions in a parallel plate flow chamber, and the other employs flow cytometry to examine the specificity in ligand binding and neutrophil activation in suspension. Evidence suggests the activation of neutrophils rolling on E-selectin that is independent of signaling through G-proteins. Neutrophil emigration appears to be regulated by shear and signals transduced through selectins during tethering and rolling.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages57
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Selectins
E-Selectin
Shear flow
Integrins
Chemical activation
Flow cytometry
GTP-Binding Proteins
Adhesives
Suspensions
Hydrodynamics
Ligands
Proteins

ASJC Scopus subject areas

  • Bioengineering

Cite this

Simon, S. I., Hu, Y., & Smith, C. W. (1999). Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 57). Piscataway, NJ, United States: IEEE.

Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow. / Simon, Scott I.; Hu, Yu; Smith, C. Wayne.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 57.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Simon, SI, Hu, Y & Smith, CW 1999, Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Piscataway, NJ, United States, pp. 57, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Simon SI, Hu Y, Smith CW. Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. Piscataway, NJ, United States: IEEE. 1999. p. 57
Simon, Scott I. ; Hu, Yu ; Smith, C. Wayne. / Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. pp. 57
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