Abstract
The functional significance of E-selectin binding to neutrophils under shear flow in the absence of chemotactic stimulation is examined. Two experimental settings were used. One simulates the potential influence of hydrodynamic shear on intercellular adhesive interactions in a parallel plate flow chamber, and the other employs flow cytometry to examine the specificity in ligand binding and neutrophil activation in suspension. Evidence suggests the activation of neutrophils rolling on E-selectin that is independent of signaling through G-proteins. Neutrophil emigration appears to be regulated by shear and signals transduced through selectins during tethering and rolling.
| Original language | English (US) |
|---|---|
| Title of host publication | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Place of Publication | Piscataway, NJ, United States |
| Publisher | IEEE |
| Pages | 57 |
| Number of pages | 1 |
| Volume | 1 |
| ISBN (Print) | 0780356756 |
| State | Published - 1999 |
| Externally published | Yes |
| Event | Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA Duration: Oct 13 1999 → Oct 16 1999 |
Other
| Other | Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) |
|---|---|
| City | Atlanta, GA, USA |
| Period | 10/13/99 → 10/16/99 |
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ASJC Scopus subject areas
- Bioengineering
Cite this
Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow. / Simon, Scott I.; Hu, Yu; Smith, C. Wayne.
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 57.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow
AU - Simon, Scott I.
AU - Hu, Yu
AU - Smith, C. Wayne
PY - 1999
Y1 - 1999
N2 - The functional significance of E-selectin binding to neutrophils under shear flow in the absence of chemotactic stimulation is examined. Two experimental settings were used. One simulates the potential influence of hydrodynamic shear on intercellular adhesive interactions in a parallel plate flow chamber, and the other employs flow cytometry to examine the specificity in ligand binding and neutrophil activation in suspension. Evidence suggests the activation of neutrophils rolling on E-selectin that is independent of signaling through G-proteins. Neutrophil emigration appears to be regulated by shear and signals transduced through selectins during tethering and rolling.
AB - The functional significance of E-selectin binding to neutrophils under shear flow in the absence of chemotactic stimulation is examined. Two experimental settings were used. One simulates the potential influence of hydrodynamic shear on intercellular adhesive interactions in a parallel plate flow chamber, and the other employs flow cytometry to examine the specificity in ligand binding and neutrophil activation in suspension. Evidence suggests the activation of neutrophils rolling on E-selectin that is independent of signaling through G-proteins. Neutrophil emigration appears to be regulated by shear and signals transduced through selectins during tethering and rolling.
UR - http://www.scopus.com/inward/record.url?scp=0033328802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033328802&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0033328802
SN - 0780356756
VL - 1
SP - 57
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
CY - Piscataway, NJ, United States
ER -