Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain

Aigang Lu, Joseph F. Clark, Joseph P. Broderick, Gail J. Pyne-Geithman, Kenneth R. Wagner, Pooja Khatri, Thomas Tomsick, Frank R Sharp

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A major complication of recanalization therapy after an acute arterial occlusion in brain is hemorrhagic transformation (HT). Although it is known that prolonged ischemia is important in the development of HT, the role of reperfusion in ischemia-reperfusion induced HT is less well studied. To address the effect of reperfusion on HT, we assessed the incidence and severity of hemorrhage in rats after 5 h of middle cerebral artery occlusion (MCAO) followed by 19-hour reperfusion compared to rats with permanent occlusion (PMCAO) at the same 24-hour time point. The incidence and amount of hemorrhage, neurological function, and mortality rates were measured. MCAO (5 h) with 19-hour reperfusion was associated with a significantly higher incidence of cortical hemorrhage compared to PMCAO (81.8% vs 18.2%, p < 0.05). Hemorrhage scores were higher in the 5-hour MCAO/reperfusion group compared to PMCAO rats (17.6 ± 11.5 vs 2.4 ± 5.3 in cortex, 20.4 ± 4.6 vs 9.7 ± 4.5 in striatum, p < 0.01). Neurological function was worse in the ischemia-reperfusion group compared to PMCAO (p < 0.05) and mortality rates were insignificantly higher in the 5-hour MCAO/reperfusion group vs PMCAO group (54.5% vs18.1%; p < 0.08). The results suggest that reperfusion after prolonged ischemia is associated with increased hemorrhagic transformation and neurological deterioration as compared to permanent ischemia. Whether pharmacological treatments prior to reperfusion attenuate post-ischemic HT requires further study.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalExperimental Neurology
Volume216
Issue number2
DOIs
StatePublished - Apr 2009

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Reperfusion
Hemorrhage
Brain
Middle Cerebral Artery Infarction
Ischemia
Incidence
Mortality
Pharmacology

Keywords

  • Animal model
  • Cerebral ischemia
  • Hemorrhage
  • Rat

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Lu, A., Clark, J. F., Broderick, J. P., Pyne-Geithman, G. J., Wagner, K. R., Khatri, P., ... Sharp, F. R. (2009). Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain. Experimental Neurology, 216(2), 407-412. https://doi.org/10.1016/j.expneurol.2008.12.020

Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain. / Lu, Aigang; Clark, Joseph F.; Broderick, Joseph P.; Pyne-Geithman, Gail J.; Wagner, Kenneth R.; Khatri, Pooja; Tomsick, Thomas; Sharp, Frank R.

In: Experimental Neurology, Vol. 216, No. 2, 04.2009, p. 407-412.

Research output: Contribution to journalArticle

Lu, A, Clark, JF, Broderick, JP, Pyne-Geithman, GJ, Wagner, KR, Khatri, P, Tomsick, T & Sharp, FR 2009, 'Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain', Experimental Neurology, vol. 216, no. 2, pp. 407-412. https://doi.org/10.1016/j.expneurol.2008.12.020
Lu A, Clark JF, Broderick JP, Pyne-Geithman GJ, Wagner KR, Khatri P et al. Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain. Experimental Neurology. 2009 Apr;216(2):407-412. https://doi.org/10.1016/j.expneurol.2008.12.020
Lu, Aigang ; Clark, Joseph F. ; Broderick, Joseph P. ; Pyne-Geithman, Gail J. ; Wagner, Kenneth R. ; Khatri, Pooja ; Tomsick, Thomas ; Sharp, Frank R. / Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain. In: Experimental Neurology. 2009 ; Vol. 216, No. 2. pp. 407-412.
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