Mechanical Disruption of Tumors by Iron Particles and Magnetic Field Application Results in Increased Anti-Tumor Immune Responses

Myriam N. Bouchlaka, Gail D. Sckisel, Danice Wilkins, Emanual Michael Maverakis, Arta M Monjazeb, Maxwell A Fung, Lisbeth Welniak, Doug Redelman, Alan Fuchs, Cahit A. Evrensel, William J Murphy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The primary tumor represents a potential source of antigens for priming immune responses for disseminated disease. Current means of debulking tumors involves the use of cytoreductive conditioning that impairs immune cells or removal by surgery. We hypothesized that activation of the immune system could occur through the localized release of tumor antigens and induction of tumor death due to physical disruption of tumor architecture and destruction of the primary tumor in situ. This was accomplished by intratumor injection of magneto-rheological fluid (MRF) consisting of iron microparticles, in Balb/c mice bearing orthotopic 4T1 breast cancer, followed by local application of a magnetic field resulting in immediate coalescence of the particles, tumor cell death, slower growth of primary tumors as well as decreased tumor progression in distant sites and metastatic spread. This treatment was associated with increased activation of DCs in the draining lymph nodes and recruitment of both DCs and CD8(+)T cells to the tumor. The particles remained within the tumor and no toxicities were observed. The immune induction observed was significantly greater compared to cryoablation. Further anti-tumor effects were observed when MRF/magnet therapy was combined with systemic low dose immunotherapy. Thus, mechanical disruption of the primary tumor with MRF/magnetic field application represents a novel means to induce systemic immune activation in cancer.

Original languageEnglish (US)
Article numbere48049
JournalPLoS One
Volume7
Issue number10
DOIs
StatePublished - Oct 25 2012

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Magnetic Fields
magnetic fields
Tumors
Iron
immune response
iron
Magnetic fields
neoplasms
Neoplasms
Chemical activation
Bearings (structural)
antigens
Fluids
T-cells
Immune system
Cryosurgery
Magnets
Neoplasm Antigens
Cell death
Fluid Therapy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mechanical Disruption of Tumors by Iron Particles and Magnetic Field Application Results in Increased Anti-Tumor Immune Responses. / Bouchlaka, Myriam N.; Sckisel, Gail D.; Wilkins, Danice; Maverakis, Emanual Michael; Monjazeb, Arta M; Fung, Maxwell A; Welniak, Lisbeth; Redelman, Doug; Fuchs, Alan; Evrensel, Cahit A.; Murphy, William J.

In: PLoS One, Vol. 7, No. 10, e48049, 25.10.2012.

Research output: Contribution to journalArticle

Bouchlaka, Myriam N. ; Sckisel, Gail D. ; Wilkins, Danice ; Maverakis, Emanual Michael ; Monjazeb, Arta M ; Fung, Maxwell A ; Welniak, Lisbeth ; Redelman, Doug ; Fuchs, Alan ; Evrensel, Cahit A. ; Murphy, William J. / Mechanical Disruption of Tumors by Iron Particles and Magnetic Field Application Results in Increased Anti-Tumor Immune Responses. In: PLoS One. 2012 ; Vol. 7, No. 10.
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