Mechanical deformation and glycosaminoglycan content changes in a rabbit annular puncture disc degeneration model

Deva D. Chan, Safdar N. Khan, Xiaojing Ye, Shane B. Curtiss, Munish C. Gupta, Eric Otto Klineberg, Corey P. Neu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Study Design.: Evaluation of degenerated intervertebral discs from a rabbit annular puncture model by using specialized magnetic resonance imaging (MRI) techniques, including displacement encoding with stimulated echoes and a fast-spin echo (DENSE-FSE) acquisition and delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Objective.: To evaluate a rabbit disc degeneration model by using various MRI techniques. To determine the displacements and strains, spin-lattice relaxation time (T1), and glycosaminoglycan (GAG) distribution of degenerated discs as compared to normal and adjacent level discs. Summary of Background Data.: Annular puncture of the intervertebral disc produces disc degeneration in rabbits. DENSE-FSE has been previously demonstrated in articular cartilage for the measurement of soft tissue displacements and strains. MRI also can measure the T1 of tissue, and dGEMRIC can quantify GAG concentration in cartilage. Methods.: In eight New Zealand white rabbits, the annulus fibrosis of a lumbar disc was punctured. After 4 weeks, the punctured and cranially adjacent motion segments were isolated for MRI and histology. MRI was used to estimate the disc volume and map T1. DENSE-FSE was used to determine displacements for the estimation of strains. dGEMRIC was then used to determine GAG distributions. Results.: Histology and standard MRI indicated degeneration in punctured discs. Disc volume increased significantly at 4 weeks after the puncture. Displacement of the nucleus pulposus was distinct from that of the annulus fibrosis in most untreated discs but not in punctured discs. T1 was significantly higher and GAG concentration significantly lower in punctured discs compared with untreated adjacent level discs. Conclusion.: Noninvasive and quantitative MRI techniques can be used to evaluate the mechanical and biochemical changes that occur with animal models of disc degeneration. DENSE-FSE, dGEMRIC, and similar techniques have potential for evaluating the progression of disc degeneration and the efficacy of treatments.

Original languageEnglish (US)
Pages (from-to)1438-1445
Number of pages8
JournalSpine
Volume36
Issue number18
DOIs
StatePublished - Aug 15 2011

Fingerprint

Intervertebral Disc Degeneration
Glycosaminoglycans
Punctures
Magnetic Resonance Imaging
Rabbits
Cartilage
Gadolinium
Intervertebral Disc
Histology
Fibrosis
Articular Cartilage
Animal Models

Keywords

  • cartilage elastography
  • dGEMRIC
  • displacement-encoded MRI
  • quantitative MRI
  • rabbit disc degeneration

ASJC Scopus subject areas

  • Clinical Neurology
  • Orthopedics and Sports Medicine

Cite this

Mechanical deformation and glycosaminoglycan content changes in a rabbit annular puncture disc degeneration model. / Chan, Deva D.; Khan, Safdar N.; Ye, Xiaojing; Curtiss, Shane B.; Gupta, Munish C.; Klineberg, Eric Otto; Neu, Corey P.

In: Spine, Vol. 36, No. 18, 15.08.2011, p. 1438-1445.

Research output: Contribution to journalArticle

Chan, Deva D. ; Khan, Safdar N. ; Ye, Xiaojing ; Curtiss, Shane B. ; Gupta, Munish C. ; Klineberg, Eric Otto ; Neu, Corey P. / Mechanical deformation and glycosaminoglycan content changes in a rabbit annular puncture disc degeneration model. In: Spine. 2011 ; Vol. 36, No. 18. pp. 1438-1445.
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AU - Chan, Deva D.

AU - Khan, Safdar N.

AU - Ye, Xiaojing

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AU - Gupta, Munish C.

AU - Klineberg, Eric Otto

AU - Neu, Corey P.

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AB - Study Design.: Evaluation of degenerated intervertebral discs from a rabbit annular puncture model by using specialized magnetic resonance imaging (MRI) techniques, including displacement encoding with stimulated echoes and a fast-spin echo (DENSE-FSE) acquisition and delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Objective.: To evaluate a rabbit disc degeneration model by using various MRI techniques. To determine the displacements and strains, spin-lattice relaxation time (T1), and glycosaminoglycan (GAG) distribution of degenerated discs as compared to normal and adjacent level discs. Summary of Background Data.: Annular puncture of the intervertebral disc produces disc degeneration in rabbits. DENSE-FSE has been previously demonstrated in articular cartilage for the measurement of soft tissue displacements and strains. MRI also can measure the T1 of tissue, and dGEMRIC can quantify GAG concentration in cartilage. Methods.: In eight New Zealand white rabbits, the annulus fibrosis of a lumbar disc was punctured. After 4 weeks, the punctured and cranially adjacent motion segments were isolated for MRI and histology. MRI was used to estimate the disc volume and map T1. DENSE-FSE was used to determine displacements for the estimation of strains. dGEMRIC was then used to determine GAG distributions. Results.: Histology and standard MRI indicated degeneration in punctured discs. Disc volume increased significantly at 4 weeks after the puncture. Displacement of the nucleus pulposus was distinct from that of the annulus fibrosis in most untreated discs but not in punctured discs. T1 was significantly higher and GAG concentration significantly lower in punctured discs compared with untreated adjacent level discs. Conclusion.: Noninvasive and quantitative MRI techniques can be used to evaluate the mechanical and biochemical changes that occur with animal models of disc degeneration. DENSE-FSE, dGEMRIC, and similar techniques have potential for evaluating the progression of disc degeneration and the efficacy of treatments.

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