Mechanical activation of dorsal root ganglion cells in vitro: Comparison with capsaicin and modulation by κ-opioids

Juergen M. Gschossmann, Victor V. Chaban, James A. McRoberts, Helen E Raybould, Steven H. Young, Helena S. Ennes, Tony Lembo, Emeran A. Mayer

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The aim of this study was to characterize plasma membrane pathways involved in the intracellular calcium ([Ca2+](i)) response of small DRG neurons to mechanical stimulation and the modulation of these pathways by κ-opioids. [Ca2+](i) responses were measured by fluorescence video microscopy of Fura-2 labeled lumbosacral DRG neurons obtained from adult rats in short-term primary culture. Transient focal mechanical stimulation of the soma, or brief superfusion with 300 nM capsaicin, resulted to [Ca2+](i) increases which were abolished in Ca2+-free solution, but unaffected by lanthanum (25 μM) or tetrodotoxin (10-6 M). 156 out of 465 neurons tested (34%) showed mechanosensitivity while 55 out of 118 neurons (47%) were capsaicin-sensitive. Ninty percent of capsaicin-sensitive neurons were mechanosensitive. Gadolinium (Gd3+; 250 μM) and amiloride (100 μM) abolished the [Ca2+](i) transient in response to mechanical stimulation, but had no effect on capsaicin-induced [Ca2+](i) transients. The κ-opioid agonists U50,488 and fedotozine showed a dose-dependent inhibition of mechanically stimulated [Ca2+](i) transients but had little effect on capsaicin-induced [Ca2+](i) transients. The inhibitory effect of U50,488 was abolished by the κ-opioid antagonist nor-Binaltorphimine dihydrochloride (nor-BNI; 100 nM), and by high concentrations of naloxone (30-100 nM), but not by low concentrations of naloxone (3 nM). We conclude that mechanically induced [Ca2+](i) transients in small diameter DRG somas are mediated by influx of Ca2+ through a Gd3+- and amiloride-sensitive plasma membrane pathway that is co-expressed with capsaicin-sensitive channels. Mechanical-, but not capsaicin-mediated, Ca2+ transients are sensitive to κ-opioid agonists. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)101-110
Number of pages10
JournalBrain Research
Volume856
Issue number1-2
DOIs
StatePublished - 2000

Fingerprint

Capsaicin
Spinal Ganglia
Opioid Analgesics
Diagnosis-Related Groups
Neurons
Amiloride
Carisoprodol
Naloxone
Cell Membrane
Lanthanum
Video Microscopy
Narcotic Antagonists
Fura-2
Tetrodotoxin
Gadolinium
In Vitro Techniques
Fluorescence Microscopy
Calcium

Keywords

  • κ-Opioid receptor
  • Capsaicin
  • Dorsal root ganglia
  • Fedotozine
  • Mechanostimulation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Gschossmann, J. M., Chaban, V. V., McRoberts, J. A., Raybould, H. E., Young, S. H., Ennes, H. S., ... Mayer, E. A. (2000). Mechanical activation of dorsal root ganglion cells in vitro: Comparison with capsaicin and modulation by κ-opioids. Brain Research, 856(1-2), 101-110. https://doi.org/10.1016/S0006-8993(99)02353-7

Mechanical activation of dorsal root ganglion cells in vitro : Comparison with capsaicin and modulation by κ-opioids. / Gschossmann, Juergen M.; Chaban, Victor V.; McRoberts, James A.; Raybould, Helen E; Young, Steven H.; Ennes, Helena S.; Lembo, Tony; Mayer, Emeran A.

In: Brain Research, Vol. 856, No. 1-2, 2000, p. 101-110.

Research output: Contribution to journalArticle

Gschossmann, JM, Chaban, VV, McRoberts, JA, Raybould, HE, Young, SH, Ennes, HS, Lembo, T & Mayer, EA 2000, 'Mechanical activation of dorsal root ganglion cells in vitro: Comparison with capsaicin and modulation by κ-opioids', Brain Research, vol. 856, no. 1-2, pp. 101-110. https://doi.org/10.1016/S0006-8993(99)02353-7
Gschossmann, Juergen M. ; Chaban, Victor V. ; McRoberts, James A. ; Raybould, Helen E ; Young, Steven H. ; Ennes, Helena S. ; Lembo, Tony ; Mayer, Emeran A. / Mechanical activation of dorsal root ganglion cells in vitro : Comparison with capsaicin and modulation by κ-opioids. In: Brain Research. 2000 ; Vol. 856, No. 1-2. pp. 101-110.
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abstract = "The aim of this study was to characterize plasma membrane pathways involved in the intracellular calcium ([Ca2+](i)) response of small DRG neurons to mechanical stimulation and the modulation of these pathways by κ-opioids. [Ca2+](i) responses were measured by fluorescence video microscopy of Fura-2 labeled lumbosacral DRG neurons obtained from adult rats in short-term primary culture. Transient focal mechanical stimulation of the soma, or brief superfusion with 300 nM capsaicin, resulted to [Ca2+](i) increases which were abolished in Ca2+-free solution, but unaffected by lanthanum (25 μM) or tetrodotoxin (10-6 M). 156 out of 465 neurons tested (34{\%}) showed mechanosensitivity while 55 out of 118 neurons (47{\%}) were capsaicin-sensitive. Ninty percent of capsaicin-sensitive neurons were mechanosensitive. Gadolinium (Gd3+; 250 μM) and amiloride (100 μM) abolished the [Ca2+](i) transient in response to mechanical stimulation, but had no effect on capsaicin-induced [Ca2+](i) transients. The κ-opioid agonists U50,488 and fedotozine showed a dose-dependent inhibition of mechanically stimulated [Ca2+](i) transients but had little effect on capsaicin-induced [Ca2+](i) transients. The inhibitory effect of U50,488 was abolished by the κ-opioid antagonist nor-Binaltorphimine dihydrochloride (nor-BNI; 100 nM), and by high concentrations of naloxone (30-100 nM), but not by low concentrations of naloxone (3 nM). We conclude that mechanically induced [Ca2+](i) transients in small diameter DRG somas are mediated by influx of Ca2+ through a Gd3+- and amiloride-sensitive plasma membrane pathway that is co-expressed with capsaicin-sensitive channels. Mechanical-, but not capsaicin-mediated, Ca2+ transients are sensitive to κ-opioid agonists. Copyright (C) 2000 Elsevier Science B.V.",
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AU - Young, Steven H.

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AU - Lembo, Tony

AU - Mayer, Emeran A.

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