Measuring intracellular oxygenation with myoglobin MRS

Research output: Contribution to journalArticle

Abstract

Despite the obvious importance of oxygen (-Acny2) in maintaining cellular viability, many fundamental questions still abound about its role in regulating metabolism. These include the knowledge of the critical O2 level required by the cell to maintain healthy function, the role played by O2 in cellular signaling, and the biochemical mechanism of intracellular O2 transport. To address such questions, biophysical techniques must first measure the intracellular oxygen level. Fortunately, detecting the 1H nuclear magnetic resonance (NMR) myoglobin (Mb) signals of valine E11 (Val-E11) γ 1-CH3 and the proximal histidine F8 (His-F8) NδH in vivo has opened unique opportunities to clarify the cellular O2 level, to understand the role of O2 in regulating respiration and oxidative phosphorylation, and to secure new insights into cellular function of Mb.

Original languageEnglish (US)
Pages (from-to)635-642
Number of pages8
JournaleMagRes
Volume4
Issue number3
DOIs
StatePublished - 2015

Keywords

  • bioenergetics
  • heart
  • hypoxia
  • ischemia
  • muscle
  • oxygen
  • respiration

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Biomedical Engineering
  • Biochemistry
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Measuring intracellular oxygenation with myoglobin MRS'. Together they form a unique fingerprint.

  • Cite this