Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion

Bernadette C. Taylor, Rory M. Brotheridge, David A. Jessup, Jeffrey L Stott

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Killer whales and sea otters maintained in captivity are the subjects of routine health monitoring programs, and interest in immunologic studies in sea otters has been rising recently in response to potential impacts from infectious disease and environmental pollution on the threatened southern sea otter population. Development of species-specific reagents for immunologic studies in these two marine mammals is currently in its infancy. In this study, killer whale and sea otter immunoglobulin-specific polyclonal antibodies were generated, and used to develop tests for serum Ig concentration in the killer whale (Orcinus orca) and the southern (Enhydra lutris nereis) and northern sea otter (Enhydra lutris lutris). Killer whale serum IgG was purified using caprylic acid/ammonium sulfate precipitation. Sea otter plasma IgG was purified using protein-A-agarose. Polyclonal anti-Ig antisera were produced in rabbits, and specificity confirmed by immunoelectrophoresis. Radial immunodiffusion was used to measure Ig concentration in serum or plasma samples derived from 21 captive killer whales, 18 wild and 4 captive southern sea otters and 15 wild and 4 captive northern sea otters grouped by age. Mean killer whale serum Ig concentration (±95% confidence interval) ranged from 15.04±3.97g/l for animals aged 0-5 years to 26.65±9.8g/l for animals aged >10 years. Mean sea otter serum Ig concentration (±95% confidence interval) ranged from 28.39±11.00g/l for southern sub-adults to 32.76±11.58g/l for southern adults. No significant difference in serum Ig concentration was found between southern and northern sea otters. Serum Ig concentrations in two northern sea otter pups were low compared to those of adult sea otters. The two serum Ig quantitation assays produced were highly specific and reproducible and will be useful additions to the limited number of tests available for immune function in these marine mammal species.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume89
Issue number3-4
DOIs
StatePublished - Oct 28 2002

Fingerprint

Killer Whale
Otters
Enhydra lutris
Orcinus orca
Immunodiffusion
blood serum
immunoglobulins
Immunoglobulins
Serum
marine mammals
confidence interval
Mammals
immunoelectrophoresis
octanoic acid
Immunoglobulin G
Confidence Intervals
infancy
polyclonal antibodies
Environmental Pollution
ammonium sulfate

Keywords

  • Immunoglobulin
  • Killer whale
  • Sea otter

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion. / Taylor, Bernadette C.; Brotheridge, Rory M.; Jessup, David A.; Stott, Jeffrey L.

In: Veterinary Immunology and Immunopathology, Vol. 89, No. 3-4, 28.10.2002, p. 187-195.

Research output: Contribution to journalArticle

Taylor, Bernadette C. ; Brotheridge, Rory M. ; Jessup, David A. ; Stott, Jeffrey L. / Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion. In: Veterinary Immunology and Immunopathology. 2002 ; Vol. 89, No. 3-4. pp. 187-195.
@article{4b836476f04d477487304209c0a906af,
title = "Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion",
abstract = "Killer whales and sea otters maintained in captivity are the subjects of routine health monitoring programs, and interest in immunologic studies in sea otters has been rising recently in response to potential impacts from infectious disease and environmental pollution on the threatened southern sea otter population. Development of species-specific reagents for immunologic studies in these two marine mammals is currently in its infancy. In this study, killer whale and sea otter immunoglobulin-specific polyclonal antibodies were generated, and used to develop tests for serum Ig concentration in the killer whale (Orcinus orca) and the southern (Enhydra lutris nereis) and northern sea otter (Enhydra lutris lutris). Killer whale serum IgG was purified using caprylic acid/ammonium sulfate precipitation. Sea otter plasma IgG was purified using protein-A-agarose. Polyclonal anti-Ig antisera were produced in rabbits, and specificity confirmed by immunoelectrophoresis. Radial immunodiffusion was used to measure Ig concentration in serum or plasma samples derived from 21 captive killer whales, 18 wild and 4 captive southern sea otters and 15 wild and 4 captive northern sea otters grouped by age. Mean killer whale serum Ig concentration (±95{\%} confidence interval) ranged from 15.04±3.97g/l for animals aged 0-5 years to 26.65±9.8g/l for animals aged >10 years. Mean sea otter serum Ig concentration (±95{\%} confidence interval) ranged from 28.39±11.00g/l for southern sub-adults to 32.76±11.58g/l for southern adults. No significant difference in serum Ig concentration was found between southern and northern sea otters. Serum Ig concentrations in two northern sea otter pups were low compared to those of adult sea otters. The two serum Ig quantitation assays produced were highly specific and reproducible and will be useful additions to the limited number of tests available for immune function in these marine mammal species.",
keywords = "Immunoglobulin, Killer whale, Sea otter",
author = "Taylor, {Bernadette C.} and Brotheridge, {Rory M.} and Jessup, {David A.} and Stott, {Jeffrey L}",
year = "2002",
month = "10",
day = "28",
doi = "10.1016/S0165-2427(02)00208-8",
language = "English (US)",
volume = "89",
pages = "187--195",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion

AU - Taylor, Bernadette C.

AU - Brotheridge, Rory M.

AU - Jessup, David A.

AU - Stott, Jeffrey L

PY - 2002/10/28

Y1 - 2002/10/28

N2 - Killer whales and sea otters maintained in captivity are the subjects of routine health monitoring programs, and interest in immunologic studies in sea otters has been rising recently in response to potential impacts from infectious disease and environmental pollution on the threatened southern sea otter population. Development of species-specific reagents for immunologic studies in these two marine mammals is currently in its infancy. In this study, killer whale and sea otter immunoglobulin-specific polyclonal antibodies were generated, and used to develop tests for serum Ig concentration in the killer whale (Orcinus orca) and the southern (Enhydra lutris nereis) and northern sea otter (Enhydra lutris lutris). Killer whale serum IgG was purified using caprylic acid/ammonium sulfate precipitation. Sea otter plasma IgG was purified using protein-A-agarose. Polyclonal anti-Ig antisera were produced in rabbits, and specificity confirmed by immunoelectrophoresis. Radial immunodiffusion was used to measure Ig concentration in serum or plasma samples derived from 21 captive killer whales, 18 wild and 4 captive southern sea otters and 15 wild and 4 captive northern sea otters grouped by age. Mean killer whale serum Ig concentration (±95% confidence interval) ranged from 15.04±3.97g/l for animals aged 0-5 years to 26.65±9.8g/l for animals aged >10 years. Mean sea otter serum Ig concentration (±95% confidence interval) ranged from 28.39±11.00g/l for southern sub-adults to 32.76±11.58g/l for southern adults. No significant difference in serum Ig concentration was found between southern and northern sea otters. Serum Ig concentrations in two northern sea otter pups were low compared to those of adult sea otters. The two serum Ig quantitation assays produced were highly specific and reproducible and will be useful additions to the limited number of tests available for immune function in these marine mammal species.

AB - Killer whales and sea otters maintained in captivity are the subjects of routine health monitoring programs, and interest in immunologic studies in sea otters has been rising recently in response to potential impacts from infectious disease and environmental pollution on the threatened southern sea otter population. Development of species-specific reagents for immunologic studies in these two marine mammals is currently in its infancy. In this study, killer whale and sea otter immunoglobulin-specific polyclonal antibodies were generated, and used to develop tests for serum Ig concentration in the killer whale (Orcinus orca) and the southern (Enhydra lutris nereis) and northern sea otter (Enhydra lutris lutris). Killer whale serum IgG was purified using caprylic acid/ammonium sulfate precipitation. Sea otter plasma IgG was purified using protein-A-agarose. Polyclonal anti-Ig antisera were produced in rabbits, and specificity confirmed by immunoelectrophoresis. Radial immunodiffusion was used to measure Ig concentration in serum or plasma samples derived from 21 captive killer whales, 18 wild and 4 captive southern sea otters and 15 wild and 4 captive northern sea otters grouped by age. Mean killer whale serum Ig concentration (±95% confidence interval) ranged from 15.04±3.97g/l for animals aged 0-5 years to 26.65±9.8g/l for animals aged >10 years. Mean sea otter serum Ig concentration (±95% confidence interval) ranged from 28.39±11.00g/l for southern sub-adults to 32.76±11.58g/l for southern adults. No significant difference in serum Ig concentration was found between southern and northern sea otters. Serum Ig concentrations in two northern sea otter pups were low compared to those of adult sea otters. The two serum Ig quantitation assays produced were highly specific and reproducible and will be useful additions to the limited number of tests available for immune function in these marine mammal species.

KW - Immunoglobulin

KW - Killer whale

KW - Sea otter

UR - http://www.scopus.com/inward/record.url?scp=0037191050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037191050&partnerID=8YFLogxK

U2 - 10.1016/S0165-2427(02)00208-8

DO - 10.1016/S0165-2427(02)00208-8

M3 - Article

C2 - 12383650

AN - SCOPUS:0037191050

VL - 89

SP - 187

EP - 195

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 3-4

ER -