Measurement of expression of the HSP70 protein family.

M. Bergeron, Frank R Sharp

Research output: Contribution to journalArticle

Abstract

The heat shock protein family of proteins include a number of proteins of similar sizes that are inducible by a wide variety of "stress" stimuli including heat shock, ischemia, toxic metals, amino acid analogs, and some chemotherapeutic and anesthetic chemicals. The functions of these proteins are diverse but are generally associated with molecular chaperoning of proteins. This unit contains a wide variety of protocols for analyzing expression of this family at the transcriptional and translational level: northern blot hybridization, in situ hybridization, metabolic labeling and SDS-PAGE, and immunoblotting.

Original languageEnglish (US)
JournalCurrent protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
VolumeChapter 2
StatePublished - May 2001
Externally publishedYes

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In Situ Hybridization
Proteins
Poisons
Septic Shock
Heat-Shock Proteins
Immunoblotting
Northern Blotting
Labeling
Anesthetics
Polyacrylamide Gel Electrophoresis
Ischemia
Hot Temperature
Metals
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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