Measurement of expression of the HSP70 protein family.

M. Bergeron, Frank R Sharp

Research output: Contribution to journalArticlepeer-review

Abstract

The heat shock protein family of proteins include a number of proteins of similar sizes that are inducible by a wide variety of "stress" stimuli including heat shock, ischemia, toxic metals, amino acid analogs, and some chemotherapeutic and anesthetic chemicals. The functions of these proteins are diverse but are generally associated with molecular chaperoning of proteins. This unit contains a wide variety of protocols for analyzing expression of this family at the transcriptional and translational level: northern blot hybridization, in situ hybridization, metabolic labeling and SDS-PAGE, and immunoblotting.

Original languageEnglish (US)
JournalCurrent protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
VolumeChapter 2
StatePublished - May 2001
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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