Improper handling of specimens results in artifactually high Mean Platelet Volume (MPV) measurements limiting their usefulness as a clinical tool. MPV measurement and Scanning Electron Microscopy (SEM) were performed on split specimens collected from normal dogs using two anticoagulants and two temperatures over a period of 4 hours. Platelets exposed to EDTA and maintained at 4 °C (39.2 °F) exhibited the highest artifactual increase in MPV, while those exposed to citrate and maintained at 37°C (98.6 °F) exhibited minimal change. The increase in MPV was accompanied by platelet shape change from a smooth disc to an irregular sphere with filopodia. It is recommended that citrated specimens maintained at 37°C be used in all MPV measurements.
|Original language||English (US)|
|Number of pages||5|
|Journal||Veterinary Clinical Pathology|
|State||Published - 1986|
- mean platelet volume
- storage temperature.
ASJC Scopus subject areas