Mean Platelet Volume Artifacts: The Effect of Anticoagulants and Temperature on Canine Platelets

Prem Handagama, Bernard Feldman, Constance Kono, Thomas B Farver

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Improper handling of specimens results in artifactually high Mean Platelet Volume (MPV) measurements limiting their usefulness as a clinical tool. MPV measurement and Scanning Electron Microscopy (SEM) were performed on split specimens collected from normal dogs using two anticoagulants and two temperatures over a period of 4 hours. Platelets exposed to EDTA and maintained at 4 °C (39.2 °F) exhibited the highest artifactual increase in MPV, while those exposed to citrate and maintained at 37°C (98.6 °F) exhibited minimal change. The increase in MPV was accompanied by platelet shape change from a smooth disc to an irregular sphere with filopodia. It is recommended that citrated specimens maintained at 37°C be used in all MPV measurements.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalVeterinary Clinical Pathology
Volume15
Issue number4
DOIs
StatePublished - 1986

Fingerprint

Mean Platelet Volume
anticoagulant activity
Artifacts
Anticoagulants
Canidae
Blood Platelets
Temperature
dogs
temperature
pseudopodia
Specimen Handling
Pseudopodia
anticoagulants
Edetic Acid
Citric Acid
citrates
Electron Scanning Microscopy
scanning electron microscopy
Dogs

Keywords

  • anticoagulant
  • mean platelet volume
  • platelets
  • storage temperature.

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Mean Platelet Volume Artifacts : The Effect of Anticoagulants and Temperature on Canine Platelets. / Handagama, Prem; Feldman, Bernard; Kono, Constance; Farver, Thomas B.

In: Veterinary Clinical Pathology, Vol. 15, No. 4, 1986, p. 13-17.

Research output: Contribution to journalArticle

Handagama, Prem ; Feldman, Bernard ; Kono, Constance ; Farver, Thomas B. / Mean Platelet Volume Artifacts : The Effect of Anticoagulants and Temperature on Canine Platelets. In: Veterinary Clinical Pathology. 1986 ; Vol. 15, No. 4. pp. 13-17.
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