Mean Platelet Volume Artifacts: The Effect of Anticoagulants and Temperature on Canine Platelets

Prem Handagama, Bernard Feldman, Constance Kono, Thomas B Farver

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19 Scopus citations


Improper handling of specimens results in artifactually high Mean Platelet Volume (MPV) measurements limiting their usefulness as a clinical tool. MPV measurement and Scanning Electron Microscopy (SEM) were performed on split specimens collected from normal dogs using two anticoagulants and two temperatures over a period of 4 hours. Platelets exposed to EDTA and maintained at 4 °C (39.2 °F) exhibited the highest artifactual increase in MPV, while those exposed to citrate and maintained at 37°C (98.6 °F) exhibited minimal change. The increase in MPV was accompanied by platelet shape change from a smooth disc to an irregular sphere with filopodia. It is recommended that citrated specimens maintained at 37°C be used in all MPV measurements.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalVeterinary Clinical Pathology
Issue number4
StatePublished - 1986



  • anticoagulant
  • mean platelet volume
  • platelets
  • storage temperature.

ASJC Scopus subject areas

  • veterinary(all)

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