MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer

N. Dodge Zantek, J. Walker-Daniels, J. Stewart, R. K. Hansen, D. Robinson, H. Miao, B. Wang, Hsing-Jien Kung, M. J. Bissell, M. S. Kinch

Research output: Contribution to journalArticle

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Abstract

Purpose: There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. Experimental Design: We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. Results: NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. Conclusions: MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.

Original languageEnglish (US)
Pages (from-to)3640-3648
Number of pages9
JournalClinical Cancer Research
Volume7
Issue number11
StatePublished - 2001

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Cell Communication
Breast Neoplasms
Cell Adhesion
Protein-Tyrosine Kinases
EphA2 Receptor
Cadherins
Signal Transduction
Research Design
Membranes
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dodge Zantek, N., Walker-Daniels, J., Stewart, J., Hansen, R. K., Robinson, D., Miao, H., ... Kinch, M. S. (2001). MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer. Clinical Cancer Research, 7(11), 3640-3648.

MCF-10A-NeoST : A new cell system for studying cell-ECM and cell-cell interactions in breast cancer. / Dodge Zantek, N.; Walker-Daniels, J.; Stewart, J.; Hansen, R. K.; Robinson, D.; Miao, H.; Wang, B.; Kung, Hsing-Jien; Bissell, M. J.; Kinch, M. S.

In: Clinical Cancer Research, Vol. 7, No. 11, 2001, p. 3640-3648.

Research output: Contribution to journalArticle

Dodge Zantek, N, Walker-Daniels, J, Stewart, J, Hansen, RK, Robinson, D, Miao, H, Wang, B, Kung, H-J, Bissell, MJ & Kinch, MS 2001, 'MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer', Clinical Cancer Research, vol. 7, no. 11, pp. 3640-3648.
Dodge Zantek N, Walker-Daniels J, Stewart J, Hansen RK, Robinson D, Miao H et al. MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer. Clinical Cancer Research. 2001;7(11):3640-3648.
Dodge Zantek, N. ; Walker-Daniels, J. ; Stewart, J. ; Hansen, R. K. ; Robinson, D. ; Miao, H. ; Wang, B. ; Kung, Hsing-Jien ; Bissell, M. J. ; Kinch, M. S. / MCF-10A-NeoST : A new cell system for studying cell-ECM and cell-cell interactions in breast cancer. In: Clinical Cancer Research. 2001 ; Vol. 7, No. 11. pp. 3640-3648.
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