Abstract
We have cloned the cDNA encoding human liver glycogen phosphorylase (glycogenosis type VI) from a fetal brain cDNA library. Liver (L) and muscle (M) phosphorylase cDNA probes were used to determine the relative abundance of mRNA encoding the L- and M-isozymes of phosphorylase in human fetal and adult tissues. The transcript encoding the M-isozyme is 3.4 kb; the L-isozyme transcript is 3.3 kb. Transcriptional expression of the L-isozyme in human and primate tissues was found to differ from the isozyme's reported tissue specificity in non-primate mammals. Furthermore, using degenerate oligonucleotide probes to two different coding regions of M-phosphorylase, a novel 4.1-kb transcript was demonstrated to be present in human fetal and adult brain.
Original language | English (US) |
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Pages (from-to) | 293-308 |
Number of pages | 16 |
Journal | Journal of Neurogenetics |
Volume | 4 |
Issue number | 1 |
DOIs | |
State | Published - 1987 |
Keywords
- Glycogen phosphorylase isozyme
- Hers' disease
- Human and primate tissues
- McArdle's disease
- Transcriptional expression
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Genetics
- Developmental Biology
- Neuroscience(all)